Formation and purification of tailored liposomes for drug delivery using a module-based micro continuous-flow system

Nikolay Dimov, Elisabeth Kastner, Maryam Hussain, Yvonne Perrie, Nicolas Szita

Research output: Contribution to journalArticle

Abstract

Liposomes are lipid based bilayer vesicles that can encapsulate, deliver and release low-soluble drugs and small molecules to a specific target site in the body. They are currently exploited in several nanomedicine formulations. However, their development and application is still limited by expensive and time-consuming process development and production methods. Therefore, to exploit these systems more effectively and support the rapid translation of new liposomal nanomedicines from bench to bedside, new cost-effective and scalable production methods are needed. We present a continuous process flow system for the preparation, modification and purification of liposomes which offers lab-on-chip scale production. The system was evaluated for a range of small vesicles (below 300 nm) varying in lipid composition, size and charge; it offers effective and rapid nanomedicine purification with high lipid recovery (> 98%) combined with effective removal of non-entrapped drug (propofol >95% reduction of non-entrapped drug present) or protein (ovalbumin >90% reduction of OVA present) and organic solvent (ethanol >95% reduction) in less than 4 minutes. The key advantages of using this bench-top, rapid, process development tool are the flexible operating conditions, interchangeable membranes and scalable high-throughput yields, thereby offering simultaneous manufacturing and purification of nanoparticles with tailored surface attributes.

LanguageEnglish
Article number12045
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 21 Sep 2017

Fingerprint

Nanomedicine
Liposomes
Pharmaceutical Preparations
Lipids
Ovalbumin
Lipid Bilayers
Propofol
Nanoparticles
Ethanol
Costs and Cost Analysis
Membranes
Proteins

Bibliographical note

Copyright: The authors. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Funding: EPSRC Centre for Innovative Manufacturing in Emergent Macromolecular Therapies and Aston University. BBSRC (BB/L000997/1) and the European Research Area initiative on industrial biotechnology (ERA-IB; third joint call).

Keywords

  • Drug delivery
  • Microfluidics
  • Biomedical engineering
  • Drug development

Cite this

Dimov, Nikolay ; Kastner, Elisabeth ; Hussain, Maryam ; Perrie, Yvonne ; Szita, Nicolas. / Formation and purification of tailored liposomes for drug delivery using a module-based micro continuous-flow system. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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Formation and purification of tailored liposomes for drug delivery using a module-based micro continuous-flow system. / Dimov, Nikolay; Kastner, Elisabeth; Hussain, Maryam; Perrie, Yvonne; Szita, Nicolas.

In: Scientific Reports, Vol. 7, No. 1, 12045, 21.09.2017.

Research output: Contribution to journalArticle

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