Free radical reactions in atherosclerosis; An EPR spectrometry study

F. A. Taiwo*, H. J. Powers, E. Nakano, Helen R. Griffiths, D. F. Nugent

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

The copper catalysed oxidation of homocysteine has been studied by electron paramagnetic resonance (EPR) spectroscopy and spin trapping techniques to determine the nature of free radical species formed under varying experimental conditions. Three radicals; thiyl, alkyl and hydroxyl were detected with hydroxyl being predominant. A reaction mechanism is proposed involving Fenton chemistry. Inclusion of catalase to test for intermediate generation of hydrogen peroxide showed a marked reduction in amount of hydroxyl radical generated. In contrast, the addition of superoxide dismutase showed no significant effect on the level of hydroxyl radical formed. Enhanced radical formation was observed at higher levels of oxygen, an effect which has consequences for differential oxygen levels in arterial and venous systems. Implications are drawn for a higher incidence of atherosclerotic plaque formation in arteries versus veins. © 2006 - IOS Press and the authors. All rights reserved.

Original languageEnglish
Pages (from-to)67-80
Number of pages14
JournalSpectroscopy
Volume20
Issue number2
DOIs
Publication statusPublished - 18 May 2006

Fingerprint

Free radical reactions
Hydroxyl Radical
Spectrometry
Paramagnetic resonance
Oxygen
Homocysteine
Catalase
Hydrogen Peroxide
Superoxide Dismutase
Free Radicals
Copper
Spectroscopy
Oxidation

Keywords

  • Atherosclerosis
  • Copper
  • EPR
  • Homocysteine
  • Hydroxyl radicals
  • LDL

Cite this

Taiwo, F. A., Powers, H. J., Nakano, E., Griffiths, H. R., & Nugent, D. F. (2006). Free radical reactions in atherosclerosis; An EPR spectrometry study. Spectroscopy, 20(2), 67-80. https://doi.org/10.1155/2006/474183
Taiwo, F. A. ; Powers, H. J. ; Nakano, E. ; Griffiths, Helen R. ; Nugent, D. F. / Free radical reactions in atherosclerosis; An EPR spectrometry study. In: Spectroscopy. 2006 ; Vol. 20, No. 2. pp. 67-80.
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Taiwo, FA, Powers, HJ, Nakano, E, Griffiths, HR & Nugent, DF 2006, 'Free radical reactions in atherosclerosis; An EPR spectrometry study', Spectroscopy, vol. 20, no. 2, pp. 67-80. https://doi.org/10.1155/2006/474183

Free radical reactions in atherosclerosis; An EPR spectrometry study. / Taiwo, F. A.; Powers, H. J.; Nakano, E.; Griffiths, Helen R.; Nugent, D. F.

In: Spectroscopy, Vol. 20, No. 2, 18.05.2006, p. 67-80.

Research output: Contribution to journalArticle

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Taiwo FA, Powers HJ, Nakano E, Griffiths HR, Nugent DF. Free radical reactions in atherosclerosis; An EPR spectrometry study. Spectroscopy. 2006 May 18;20(2):67-80. https://doi.org/10.1155/2006/474183