Free radicals and redox signalling in T-cells during chronic inflammation and ageing

Helen R Griffiths, Christopher R Dunston, Stuart J Bennett, Melissa M. Grant, Darren C. Phillips, George D. Kitas

Research output: Contribution to journalArticle

Abstract

During chronic inflammation and ageing, the increase in oxidative stress in both intracellular and extracellular compartments is likely to influence local cell functions. Redox changes alter the T-cell proteome in a quantitative and qualitative manner, and post-translational modifications to surface and cytoplasmic proteins by increased reactive species can influence T-cell function. Previously, we have shown that RA (rheumatoid arthritis) T-cells exhibit reduced ROS (reactive oxygen species) production in response to extracellular stimulation compared with age-matched controls, and basal ROS levels [measured as DCF (2',7'-dichlorofluorescein) fluorescence] are lower in RA T-cells. In contrast, exposing T-cells in vitro to different extracellular redox environments modulates intracellular signalling and enhances cytokine secretion. Together, these data suggest that a complex relationship exists between intra- and extra-cellular redox compartments which contribute to the T-cell phenotype.
Original languageEnglish
Pages (from-to)1273-1278
Number of pages6
JournalBiochemical Society Transactions
Volume39
Issue number5
DOIs
Publication statusPublished - Oct 2011

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T-cells
Oxidation-Reduction
Free Radicals
Aging of materials
Inflammation
T-Lymphocytes
Reactive Oxygen Species
Rheumatoid Arthritis
Oxidative stress
Proteome
Post Translational Protein Processing
Membrane Proteins
Oxidative Stress
Fluorescence
Cytokines
Phenotype
Proteins

Keywords

  • aging
  • free radicals
  • humans
  • inflammation
  • oxidation-reduction
  • oxidative stress
  • reactive oxygen species
  • signal transduction
  • T-lymphocytes

Cite this

Griffiths, H. R., Dunston, C. R., Bennett, S. J., Grant, M. M., Phillips, D. C., & Kitas, G. D. (2011). Free radicals and redox signalling in T-cells during chronic inflammation and ageing. Biochemical Society Transactions, 39(5), 1273-1278. https://doi.org/10.1042/BST0391273
Griffiths, Helen R ; Dunston, Christopher R ; Bennett, Stuart J ; Grant, Melissa M. ; Phillips, Darren C. ; Kitas, George D. / Free radicals and redox signalling in T-cells during chronic inflammation and ageing. In: Biochemical Society Transactions. 2011 ; Vol. 39, No. 5. pp. 1273-1278.
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Griffiths, HR, Dunston, CR, Bennett, SJ, Grant, MM, Phillips, DC & Kitas, GD 2011, 'Free radicals and redox signalling in T-cells during chronic inflammation and ageing', Biochemical Society Transactions, vol. 39, no. 5, pp. 1273-1278. https://doi.org/10.1042/BST0391273

Free radicals and redox signalling in T-cells during chronic inflammation and ageing. / Griffiths, Helen R; Dunston, Christopher R; Bennett, Stuart J; Grant, Melissa M.; Phillips, Darren C.; Kitas, George D.

In: Biochemical Society Transactions, Vol. 39, No. 5, 10.2011, p. 1273-1278.

Research output: Contribution to journalArticle

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Griffiths HR, Dunston CR, Bennett SJ, Grant MM, Phillips DC, Kitas GD. Free radicals and redox signalling in T-cells during chronic inflammation and ageing. Biochemical Society Transactions. 2011 Oct;39(5):1273-1278. https://doi.org/10.1042/BST0391273