Fucoidan-Mimetic Glycopolymers as Tools for Studying Molecular and Cellular Responses in Human Blood Platelets

Mattias Tengdelius, Caroline Kardeby, Knut Fälker, May Griffith, Peter Påhlsson, Peter Konradsson, Magnus Grenegård

Research output: Contribution to journalArticlepeer-review

Abstract

The marine sulfated polysaccharide fucoidan displays superior ability to induce platelet aggregation compared to other sulfated polysaccharides. As such, it is an attractive tool for studying molecular and cellular responses in activated platelets. The heterogeneous structure, however, poses a problem in such applications. This study describes the synthesis of sulfated α-l-fucoside-pendant poly(methacryl amides) with homogeneous structures. By using both thiol-mediated chain transfer and reversible addition-fragmentation chain transfer polymerization techniques, glycopolymers with different chain lengths are obtained. These glycopolymers show platelet aggregation response and surface changes similar to those of fucoidan, and cause platelet activation through intracellular signaling as shown by extensive protein tyrosine phosphorylation. As the platelet activating properties of the glycopolymers strongly mimic those of fucoidan, this study concludes these fucoidan-mimetic glycopolymers are unique tools for studying molecular and cellular responses in human blood platelets.

Original languageEnglish
Article number1600257
Number of pages9
JournalMacromolecular Bioscience
Volume17
Issue number2
DOIs
Publication statusE-pub ahead of print - 15 Feb 2017

Bibliographical note

© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords

  • biological applications of polymers
  • biomimetic
  • radical polymerization
  • reversible addition fragmentation chain transfer
  • structure-property relations

Fingerprint

Dive into the research topics of 'Fucoidan-Mimetic Glycopolymers as Tools for Studying Molecular and Cellular Responses in Human Blood Platelets'. Together they form a unique fingerprint.

Cite this