Functional analysis of heterologous GPCR signalling pathways in yeast

Graham Ladds, Alan Goddard, John Davey*

*Corresponding author for this work

Research output: Contribution to journalReview article

Abstract

G protein-coupled receptors (GPCRs) regulate diverse biological processes in eukaryotes and such conservation allows an almost unrestricted interchange of signalling components between different cell types. Yeasts are attractive hosts in which to study GPCRs - they are amenable to both genetic and biochemical manipulation and their robustness, low cost and our ability to create strains that lack endogenous GPCRs make them ideal starting points for the development of assays suitable for high-throughput screening. Here we introduce readers to the possibilities of using yeast to analyse GPCRs describing the endogenous signalling pathways, the development of assays for heterologous GPCRs and the technology to elucidate GPCR structure and activity, focusing on the budding yeast Saccharomyces cerevisiae and recent developments using the fission yeast Schizosaccharomyces pombe.

Original languageEnglish
Pages (from-to)367-373
Number of pages7
JournalTrends in Biotechnology
Volume23
Issue number7
DOIs
Publication statusPublished - Jul 2005

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