Gingipains from Porphyromonas gingivalis increase the chemotactic and respiratory burst-priming properties of the 77-amino-acid interleukin-8 variant

Irundika H.K. Dias, Lindsay Marshall, Peter A. Lambert, Iain L.C. Chapple, John B. Matthews, Helen R. Griffiths

Research output: Contribution to journalArticle

Abstract

Porphyromonas gingivalis, a gram-negative anaerobe which is implicated in the etiology of active periodontitis, secretes degradative enzymes (gingipains) and sheds proinflammatory mediators (e.g., lipopolysaccharides [LPS]). LPS triggers the secretion of interleukin-8 (IL-8) from immune (72-amino-acid [aa] variant [IL-8(72aa)]) and nonimmune (IL-8(77aa)) cells. IL-8(77aa) has low chemotactic and respiratory burst-inducing activity but is susceptible to cleavage by gingipains. This study shows that both R- and K-gingipain treatments of IL-8(77aa) significantly enhance burst activation by fMLP and chemotactic activity (P < 0.05) but decrease burst activation and chemotactic activity of IL-8(72aa) toward neutrophil-like HL60 cells and primary neutrophils (P < 0.05). Using tandem mass spectrometry, we have demonstrated that R-gingipain cleaves 5- and 11-aa peptides from the N-terminal portion of IL-8(77aa) and the resultant peptides are biologically active, while K-gingipain removes an 8-aa N-terminal peptide yielding a 69-aa isoform of IL-8 that shows enhanced biological activity. During periodontitis, secreted gingipains may differentially affect neutrophil chemotaxis and activation in response to IL-8 according to the cellular source of the chemokine.
LanguageEnglish
Pages317-323
Number of pages7
JournalInfection and Immunity
Volume76
Issue number1
DOIs
Publication statusPublished - Jan 2008

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Porphyromonas gingivalis
Respiratory Burst
Interleukin-8
Amino Acids
Periodontitis
Peptides
Lipopolysaccharides
Neutrophils
Porphyromonas gingivalis argingipain
Neutrophil Activation
HL-60 Cells
Chemotaxis
Tandem Mass Spectrometry
Chemokines
Protein Isoforms

Bibliographical note

Copyright of American Society for Microbiology

Keywords

  • Porphyromonas gingivalis
  • anaerobe
  • etiology
  • active periodontitis
  • secretes degradative enzymes
  • gingipains
  • proinflammatory mediators
  • lipopolysaccharides
  • interleukin-8
  • immune
  • variant
  • nonimmune
  • cells
  • low chemotactic
  • respiratory
  • neutrophils
  • spectrometry
  • chemokine

Cite this

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title = "Gingipains from Porphyromonas gingivalis increase the chemotactic and respiratory burst-priming properties of the 77-amino-acid interleukin-8 variant",
abstract = "Porphyromonas gingivalis, a gram-negative anaerobe which is implicated in the etiology of active periodontitis, secretes degradative enzymes (gingipains) and sheds proinflammatory mediators (e.g., lipopolysaccharides [LPS]). LPS triggers the secretion of interleukin-8 (IL-8) from immune (72-amino-acid [aa] variant [IL-8(72aa)]) and nonimmune (IL-8(77aa)) cells. IL-8(77aa) has low chemotactic and respiratory burst-inducing activity but is susceptible to cleavage by gingipains. This study shows that both R- and K-gingipain treatments of IL-8(77aa) significantly enhance burst activation by fMLP and chemotactic activity (P < 0.05) but decrease burst activation and chemotactic activity of IL-8(72aa) toward neutrophil-like HL60 cells and primary neutrophils (P < 0.05). Using tandem mass spectrometry, we have demonstrated that R-gingipain cleaves 5- and 11-aa peptides from the N-terminal portion of IL-8(77aa) and the resultant peptides are biologically active, while K-gingipain removes an 8-aa N-terminal peptide yielding a 69-aa isoform of IL-8 that shows enhanced biological activity. During periodontitis, secreted gingipains may differentially affect neutrophil chemotaxis and activation in response to IL-8 according to the cellular source of the chemokine.",
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Gingipains from Porphyromonas gingivalis increase the chemotactic and respiratory burst-priming properties of the 77-amino-acid interleukin-8 variant. / Dias, Irundika H.K.; Marshall, Lindsay; Lambert, Peter A.; Chapple, Iain L.C.; Matthews, John B.; Griffiths, Helen R.

In: Infection and Immunity, Vol. 76, No. 1, 01.2008, p. 317-323.

Research output: Contribution to journalArticle

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T1 - Gingipains from Porphyromonas gingivalis increase the chemotactic and respiratory burst-priming properties of the 77-amino-acid interleukin-8 variant

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AU - Lambert, Peter A.

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