Healthy ageing and depletion of intracellular glutathione influences T cell membrane thioredoxin-1 levels and cytokine secretion

Rita B.D. Carilho Torrão, Irundika H.K. Dias, Stuart J. Bennett, Christopher R Dunston, Helen R. Griffiths

Research output: Contribution to journalArticle

Abstract

Background: During ageing an altered redox balance has been observed in both intracellular and extracellular compartments, primarily due to glutathione depletion and metabolic stress. Maintaining redox homeostasis is important for controlling proliferation and apoptosis in response to specific stimuli for a variety of cells. For T cells, the ability to generate specific response to antigen is dependent on the oxidation state of cell surface and cytoplasmic protein-thiols. Intracellular thiols are maintained in their reduced state by a network of redox regulating peptides, proteins and enzymes such as glutathione, thioredoxins and thioredoxin reductase. Here we have investigated whether any relationship exists between age and secreted or cell surface thioredoxin-1, intracellular glutathione concentration and T cell surface thioredoxin 1 (Trx-1) and how this is related to interleukin (IL)-2 production.Results: Healthy older adults have reduced lymphocyte surface expression and lower circulating plasma Trx-1 concentrations. Using buthionine sulfoximine to deplete intracellular glutathione in Jurkat T cells we show that cell surface Trx-1 is lowered, secretion of Trx-1 is decreased and the response to the lectin phytohaemagglutinin measured as IL-2 production is also affected. These effects are recapitulated by another glutathione depleting agent, diethylmaleate.Conclusion: Together these data suggest that a relationship exists between the intracellular redox compartment and Trx-1 proteins. Loss of lymphocyte surface Trx-1 may be a useful biomarker of healthy ageing. © 2013 Carilho Torrao et al.; licensee Chemistry Central Ltd.

LanguageEnglish
Article number150
Number of pages10
JournalChemistry Central Journal
Volume7
Issue number1
DOIs
Publication statusPublished - 5 Sep 2013

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Thioredoxins
T-cells
Cell membranes
Glutathione
Aging of materials
Cytokines
diethyl maleate
Lymphocytes
Sulfhydryl Compounds
Interleukin-2
Thioredoxin-Disulfide Reductase
Buthionine Sulfoximine
Proteins
Phytohemagglutinins
Biomarkers
Lectins
Apoptosis
Plasmas
Antigens
Oxidation

Bibliographical note

© 2013 Carilho Torrao et al.; licensee Chemistry Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords

  • ageing
  • cysteine
  • membrane protein
  • redox
  • reduced glutathione
  • thioredoxin-1

Cite this

Carilho Torrão, Rita B.D. ; Dias, Irundika H.K. ; Bennett, Stuart J. ; Dunston, Christopher R ; Griffiths, Helen R. / Healthy ageing and depletion of intracellular glutathione influences T cell membrane thioredoxin-1 levels and cytokine secretion. In: Chemistry Central Journal. 2013 ; Vol. 7, No. 1.
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Healthy ageing and depletion of intracellular glutathione influences T cell membrane thioredoxin-1 levels and cytokine secretion. / Carilho Torrão, Rita B.D.; Dias, Irundika H.K.; Bennett, Stuart J.; Dunston, Christopher R; Griffiths, Helen R.

In: Chemistry Central Journal, Vol. 7, No. 1, 150, 05.09.2013.

Research output: Contribution to journalArticle

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AU - Bennett, Stuart J.

AU - Dunston, Christopher R

AU - Griffiths, Helen R.

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