High glucose and interleukin 1β-induced apoptosis in human umbilical vein endothelial cells involves in down-regulation of monocarboxylate transporter 4

Dong Wang, Qingjie Wang, Gaoliang Yan, Yong Qiao, Ling Sun, Boqian Zhu, Chengchun Tang, Yuchun Gu

Research output: Contribution to journalArticle

Abstract

Hyperglycaemia and inflammatory can induce apoptosis in vascular endothelial cells, which contributes to the development of vascular complications in diabetes. Endothelial cells depend on glycolysis for their energy metabolism, and monocarboxylate transporters (MCTs) regulate intracellular pH by mediating the influx and efflux of lactate. Here, we evaluate the role of MCT4 in high glucose (HG) and interleukin 1β (IL-1β)-induced apoptosis in human umbilical vein endothelial cells (HUVECs). We demonstrate that aortic endothelium damage is severe in db/db mice by using scanning ion conductance microscopy (SICM). HG and IL-1β decrease MCT4 and its location on plasma membrane, as well as increase lactic acid accumulation and apoptosis in HUVECs. Knockdown of MCT4 blocks lactate efflux to result in lactic acid accumulation and pH dropping, which is involved in triggering apoptosis in HUVECs.
Original languageEnglish
Pages (from-to)607-614
JournalBiochemical and Biophysical Research Communications
Volume466
Issue number4
Early online date9 Sep 2015
DOIs
Publication statusE-pub ahead of print - 9 Sep 2015

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Endothelial cells
Human Umbilical Vein Endothelial Cells
Interleukin-1
Lactic Acid
Down-Regulation
Apoptosis
Glucose
Endothelial Cells
Glycolysis
Diabetes Complications
Hyperglycemia
Energy Metabolism
Endothelium
Blood Vessels
Microscopy
Cell membranes
Medical problems
Cell Membrane
Ions
Microscopic examination

Cite this

Wang, Dong ; Wang, Qingjie ; Yan, Gaoliang ; Qiao, Yong ; Sun, Ling ; Zhu, Boqian ; Tang, Chengchun ; Gu, Yuchun. / High glucose and interleukin 1β-induced apoptosis in human umbilical vein endothelial cells involves in down-regulation of monocarboxylate transporter 4. In: Biochemical and Biophysical Research Communications. 2015 ; Vol. 466, No. 4. pp. 607-614.
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High glucose and interleukin 1β-induced apoptosis in human umbilical vein endothelial cells involves in down-regulation of monocarboxylate transporter 4. / Wang, Dong; Wang, Qingjie; Yan, Gaoliang; Qiao, Yong; Sun, Ling; Zhu, Boqian ; Tang, Chengchun; Gu, Yuchun.

In: Biochemical and Biophysical Research Communications, Vol. 466, No. 4, 09.09.2015, p. 607-614.

Research output: Contribution to journalArticle

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AU - Wang, Dong

AU - Wang, Qingjie

AU - Yan, Gaoliang

AU - Qiao, Yong

AU - Sun, Ling

AU - Zhu, Boqian

AU - Tang, Chengchun

AU - Gu, Yuchun

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AB - Hyperglycaemia and inflammatory can induce apoptosis in vascular endothelial cells, which contributes to the development of vascular complications in diabetes. Endothelial cells depend on glycolysis for their energy metabolism, and monocarboxylate transporters (MCTs) regulate intracellular pH by mediating the influx and efflux of lactate. Here, we evaluate the role of MCT4 in high glucose (HG) and interleukin 1β (IL-1β)-induced apoptosis in human umbilical vein endothelial cells (HUVECs). We demonstrate that aortic endothelium damage is severe in db/db mice by using scanning ion conductance microscopy (SICM). HG and IL-1β decrease MCT4 and its location on plasma membrane, as well as increase lactic acid accumulation and apoptosis in HUVECs. Knockdown of MCT4 blocks lactate efflux to result in lactic acid accumulation and pH dropping, which is involved in triggering apoptosis in HUVECs.

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