Hijacked then lost in translation: the plight of the recombinant host cell in membrane protein structural biology projects

Roslyn M. Bill*, Tobias von der Haar

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Membrane protein structural biology is critically dependent upon the supply of high-quality protein. Over the last few years, the value of crystallising biochemically characterised, recombinant targets that incorporate stabilising mutations has been established. Nonetheless, obtaining sufficient yields of many recombinant membrane proteins is still a major challenge. Solutions are now emerging based on an improved understanding of recombinant host cells; as a 'cell factory' each cell is tasked with managing limited resources to simultaneously balance its own growth demands with those imposed by an expression plasmid. This review examines emerging insights into the role of translation and protein folding in defining high-yielding recombinant membrane protein production in a range of host cells.

Original languageEnglish
Pages (from-to)147-155
Number of pages9
JournalCurrent Opinion in Structural Biology
Volume32
Early online date1 Jun 2015
DOIs
Publication statusPublished - Jun 2015

Bibliographical note

Funding: Biotechnology and Biological Sciences Research Council (BBSRC; grant BB/I019960/1) and the Innovative Medicines Joint Undertaking under
Grant Agreement no. 115583 to the ND4BB ENABLE Consortium. BBSRC (grant BB/I010351/1) and from the Leverhulme Trust (grant RPG-2014-032).

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