H2S regulation of nitric oxide metabolism

Gopi K. Kolluru; Shuai Yuan; Xinggui Shen; Christopher G. Kevil

doi:10.1016/bs.mie.2014.11.040

H₂S regulation of nitric oxide metabolism

Gopi K. Kolluru, Shuai Yuan, Xinggui Shen, Christopher G. Kevil

Aston Medical School

Research output: Chapter in Book/Published conference output › Chapter (peer-reviewed) › peer-review

Abstract

Nitric oxide (NO) and hydrogen sulfide (H₂S) are two major gaseous signaling molecules that regulate diverse physiological functions. Recent publications indicate the regulatory role of H₂S on NO metabolism. In this chapter, we discuss the latest findings on H₂S-NO interactions through formation of novel chemical derivatives and experimental approaches to study these adducts. This chapter also addresses potential H₂S interference on various NO detection techniques, along with precautions for analyzing biological samples from various sources. This information will facilitate critical evaluation and clearer insight into H2S regulation of NO signaling and its influence on various physiological functions.

Original language	English
Title of host publication	Hydrogen sulfide in redox biology, part A
Editors	Enrique Cadenas, Lester Packer
Place of Publication	London (UK)
Publisher	Academic Press
Pages	271-297
Number of pages	27
ISBN (Print)	978-0-12-801512-4
DOIs	https://doi.org/10.1016/bs.mie.2014.11.040
Publication status	Published - 9 Sept 2015

Publication series

Name	Methods in Enzymology
Publisher	Academics Press
Volume	554
ISSN (Print)	0076-6879

Keywords

Western blotting
cultured cells
hydrogen sulfide
fluorescent dyes
gene expression
human umbilical vein endothelial cells
luminescent measurements
signal transduction
reference standards
nitric oxide
nitric oxide synthase
real-time polymerase chain reaction

Access to Document

10.1016/bs.mie.2014.11.040

1 Chapter (peer-reviewed)

Measurement of H₂S in vivo and in vitro by the monobromobimane method
Shen, X., Kolluru, G. K., Yuan, S. & Kevil, C. G., 2015, Hydrogen sulfide in redox biology, part A. Cadenas, E. & Packer, L. (eds.). London (UK): Academic Press, p. 31-45 15 p. (Methods in Enzymology; vol. 554).
Research output: Chapter in Book/Published conference output › Chapter (peer-reviewed) › peer-review

Cite this

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title = "H2S regulation of nitric oxide metabolism",

abstract = "Nitric oxide (NO) and hydrogen sulfide (H2S) are two major gaseous signaling molecules that regulate diverse physiological functions. Recent publications indicate the regulatory role of H2S on NO metabolism. In this chapter, we discuss the latest findings on H2S-NO interactions through formation of novel chemical derivatives and experimental approaches to study these adducts. This chapter also addresses potential H2S interference on various NO detection techniques, along with precautions for analyzing biological samples from various sources. This information will facilitate critical evaluation and clearer insight into H2S regulation of NO signaling and its influence on various physiological functions.",

keywords = "Western blotting, cultured cells, hydrogen sulfide, fluorescent dyes, gene expression, human umbilical vein endothelial cells, luminescent measurements, signal transduction, reference standards, nitric oxide, nitric oxide synthase, real-time polymerase chain reaction",

author = "Kolluru, {Gopi K.} and Shuai Yuan and Xinggui Shen and Kevil, {Christopher G.}",

year = "2015",

month = sep,

day = "9",

doi = "10.1016/bs.mie.2014.11.040",

language = "English",

isbn = "978-0-12-801512-4",

series = "Methods in Enzymology",

publisher = "Academic Press",

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editor = "Enrique Cadenas and Lester Packer",

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T1 - H2S regulation of nitric oxide metabolism

AU - Kolluru, Gopi K.

AU - Yuan, Shuai

AU - Shen, Xinggui

AU - Kevil, Christopher G.

PY - 2015/9/9

Y1 - 2015/9/9

N2 - Nitric oxide (NO) and hydrogen sulfide (H2S) are two major gaseous signaling molecules that regulate diverse physiological functions. Recent publications indicate the regulatory role of H2S on NO metabolism. In this chapter, we discuss the latest findings on H2S-NO interactions through formation of novel chemical derivatives and experimental approaches to study these adducts. This chapter also addresses potential H2S interference on various NO detection techniques, along with precautions for analyzing biological samples from various sources. This information will facilitate critical evaluation and clearer insight into H2S regulation of NO signaling and its influence on various physiological functions.

AB - Nitric oxide (NO) and hydrogen sulfide (H2S) are two major gaseous signaling molecules that regulate diverse physiological functions. Recent publications indicate the regulatory role of H2S on NO metabolism. In this chapter, we discuss the latest findings on H2S-NO interactions through formation of novel chemical derivatives and experimental approaches to study these adducts. This chapter also addresses potential H2S interference on various NO detection techniques, along with precautions for analyzing biological samples from various sources. This information will facilitate critical evaluation and clearer insight into H2S regulation of NO signaling and its influence on various physiological functions.

KW - Western blotting

KW - cultured cells

KW - hydrogen sulfide

KW - fluorescent dyes

KW - gene expression

KW - human umbilical vein endothelial cells

KW - luminescent measurements

KW - signal transduction

KW - reference standards

KW - nitric oxide

KW - nitric oxide synthase

KW - real-time polymerase chain reaction

U2 - 10.1016/bs.mie.2014.11.040

DO - 10.1016/bs.mie.2014.11.040

M3 - Chapter (peer-reviewed)

C2 - 25725527

SN - 978-0-12-801512-4

T3 - Methods in Enzymology

SP - 271

EP - 297

BT - Hydrogen sulfide in redox biology, part A

A2 - Cadenas, Enrique

A2 - Packer, Lester

PB - Academic Press

CY - London (UK)

ER -

H₂S regulation of nitric oxide metabolism

Abstract

Publication series

Keywords

Access to Document

Fingerprint

Measurement of H₂S in vivo and in vitro by the monobromobimane method

Cite this

H2S regulation of nitric oxide metabolism

Abstract

Publication series

Keywords

Access to Document

Fingerprint

Research output

Measurement of H2S in vivo and in vitro by the monobromobimane method

Cite this

H₂S regulation of nitric oxide metabolism

Measurement of H₂S in vivo and in vitro by the monobromobimane method