Human adipose tissue-derived mesenchymal stem/stromal cells adhere to and inhibit the growth of Staphylococcus aureus and Pseudomonas aeruginosa

Chelsea Rheannon Wood, Douaa Al Dhahri, Ibtesam Al Delfi, Neil Anthony Pickles, Rachel L. Sammons, Tony Worthington, Karina Theresa Wright, William Eustace Basil Johnson*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

We have cultured and phenotyped human adipose tissue-derived mesenchymal stem/stromal cells (AT MSCs) and inoculated these cultures with bacteria common to infected skin wounds, i.e. Staphylococcus aureus and Pseudomonas aeruginosa. Cell interactions were examined by scanning electron microscopy (SEM), whilst bacterial growth was measured by colony forming unit (c.f.u.) and biofilm assays. AT MSCs appeared to attach to the bacteria and to engulf S. aureus. Significantly fewer bacterial c.f.u. were present in AT MSC : bacterial co-cultures compared with bacteria cultured alone. Antibacterial activity, including an inhibition of P. aeruginosa biofilm formation, was observed when bacteria were treated with conditioned medium harvested from the AT MSC : bacterial co-cultures, irrespective of the bacterial species to which the AT MSCs had been exposed to previously. Hence, we have demonstrated that AT MSCs inhibit the growth of two common bacterial species. This was associated with bacterial adhesion, potential engulfment or phagocytosis, and the secretion of antibacterial factors.

Original languageEnglish
Pages (from-to)1789-1795
Number of pages7
JournalJournal of Medical Microbiology
Volume67
Issue number12
Early online date23 Oct 2018
DOIs
Publication statusPublished - 1 Dec 2018

Keywords

  • Adipose tissue
  • Antibacterial
  • Biofilms
  • CFU assay
  • Mesenchymal stem/stromal cell (MSC)
  • SEM

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