Human heart failure biomarker immunosensor based on excessively tilted fiber gratings

Binbin Luo, Shengxi Wu, Zhonghao Zhang, Wengen Zou, Shenghui Shi, Mingfu Zhao, Nianbing Zhong, Yong Liu, Xue Zou, Lingling Wang, Weina Chai, Chuanmin Hu, Lin Zhang

Research output: Contribution to journalArticlepeer-review


A label-free immunosensor platform based on excessively tilted fiber gratings (Ex- TFGs) was developed for highly specific and fast detection of human N-terminal pro-B-type natriuretic peptide (NT-proBNP), which is considered a powerful biomarker for prognosis and risk stratification of heart failure (HF). High-purity anti-NT-proBNP monoclonal antibodies (MAbs) prepared in our laboratory were immobilized on fiber surface through the staphylococcal protein A (SPA) method for subsequent specific binding of the targeted NTproBNP. Utilizing fiber optic grating demodulation system (FOGDS), immunoassays were carried out in vitro by monitoring the resonance wavelength shift of Ex-TFG biosensor with immobilized anti-NT-proBNP MAbs. Lowest detectable concentration of ~0.5ng/mL for NTproBNP was obtained, and average sensitivity for NT-proBNP at a concentration range of 0~1.0 ng/mL was approximately 45.967 pm/(ng/mL). Several human serum samples were assessed by the proposed Ex-TFG biomarker sensor, with high specificity for NT-proBNP, indicating potential application in early diagnosing patients with acute HF symptoms.

Original languageEnglish
Pages (from-to)57-67
Number of pages11
JournalBiomedical Optics Express
Issue number1
Early online date5 Dec 2016
Publication statusPublished - 1 Jan 2017

Bibliographical note

© 2016 Optical Society of America


  • biological sensing and sensors
  • fiber optics sensors
  • medical optics and biotechnology


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