Human La is found at RNA polymerase III-transcribed genes in vivo

Jennifer A. Fairley, Theodoras Kantidakis, Niall S. Kenneth, Robert V. Intine, Richard J. Maraia, Robert J. White

Research output: Contribution to journalArticle

Abstract

The human La autoantigen can bind to nascent RNA transcripts and has also been postulated to act as an RNA polymerase III (pol III) transcription initiation and termination factor. Here, we show by chromatin immunoprecipitation (ChlP) that La is associated with pol III-transcribed genes in vivo. In contrast, the Ro autoantigen, which can also bind pol III transcripts, is not found at these genes. The putative pol III transcription factors NF1 and TFIIA are also not detected at class III genes. Binding of La remains when transcription is repressed at mitosis and does not correlate with the presence of polymerase at the gene. However, gene occupancy depends on the phosphorylation status of La, with the less prevalent, unphosphorylated form being found selectively on pol III templates.

Original languageEnglish
Pages (from-to)18350-18355
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number51
DOIs
Publication statusPublished - 20 Dec 2005

Fingerprint

RNA Polymerase III
Genes
Transcription Factor TFIIA
Peptide Initiation Factors
Chromatin Immunoprecipitation
Autoantigens
Mitosis
Phosphorylation
RNA

Keywords

  • Chromatin immunoprecipitation
  • La
  • NF1
  • RNA polymerase III transcription
  • TFIIA

Cite this

Fairley, Jennifer A. ; Kantidakis, Theodoras ; Kenneth, Niall S. ; Intine, Robert V. ; Maraia, Richard J. ; White, Robert J. / Human La is found at RNA polymerase III-transcribed genes in vivo. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 51. pp. 18350-18355.
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Human La is found at RNA polymerase III-transcribed genes in vivo. / Fairley, Jennifer A.; Kantidakis, Theodoras; Kenneth, Niall S.; Intine, Robert V.; Maraia, Richard J.; White, Robert J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 51, 20.12.2005, p. 18350-18355.

Research output: Contribution to journalArticle

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T1 - Human La is found at RNA polymerase III-transcribed genes in vivo

AU - Fairley, Jennifer A.

AU - Kantidakis, Theodoras

AU - Kenneth, Niall S.

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AB - The human La autoantigen can bind to nascent RNA transcripts and has also been postulated to act as an RNA polymerase III (pol III) transcription initiation and termination factor. Here, we show by chromatin immunoprecipitation (ChlP) that La is associated with pol III-transcribed genes in vivo. In contrast, the Ro autoantigen, which can also bind pol III transcripts, is not found at these genes. The putative pol III transcription factors NF1 and TFIIA are also not detected at class III genes. Binding of La remains when transcription is repressed at mitosis and does not correlate with the presence of polymerase at the gene. However, gene occupancy depends on the phosphorylation status of La, with the less prevalent, unphosphorylated form being found selectively on pol III templates.

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