Human rhinovirus-induced inflammatory responses are inhibited by phosphatidylserine containing liposomes

C.A. Stokes, R. Kaur, M.R. Edwards, M. Mondhe, D. Robinson, E.C. Prestwich, R.D. Hume, C.A. Marshall, Y. Perrie, V.B. O'Donnell, J.L. Harwood, I. Sabroe, L.C. Parker

Research output: Contribution to journalArticle

Abstract

Human rhinovirus (HRV) infections are major contributors to the healthcare burden associated with acute exacerbations of chronic airway disease, such as chronic obstructive pulmonary disease and asthma. Cellular responses to HRV are mediated through pattern recognition receptors that may in part signal from membrane microdomains. We previously found Toll-like receptor signaling is reduced, by targeting membrane microdomains with a specific liposomal phosphatidylserine species, 1-stearoyl-2-arachidonoyl-sn-glycero-3-phospho-L-serine (SAPS). Here we explored the ability of this approach to target a clinically important pathogen. We determined the biochemical and biophysical properties and stability of SAPS liposomes and studied their ability to modulate rhinovirus-induced inflammation, measured by cytokine production, and rhinovirus replication in both immortalized and normal primary bronchial epithelial cells. SAPS liposomes rapidly partitioned throughout the plasma membrane and internal cellular membranes of epithelial cells. Uptake of liposomes did not cause cell death, but was associated with markedly reduced inflammatory responses to rhinovirus, at the expense of only modest non-significant increases in viral replication, and without impairment of interferon receptor signaling. Thus using liposomes of phosphatidylserine to target membrane microdomains is a feasible mechanism for modulating rhinovirus-induced signaling, and potentially a prototypic new therapy for viral-mediated inflammation.

LanguageEnglish
Pages1303-1316
Number of pages14
JournalMucosal Immunology
Volume9
Issue number5
Early online date24 Feb 2016
DOIs
Publication statusPublished - Sep 2016

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Rhinovirus
Phosphatidylserines
Liposomes
Membrane Microdomains
Epithelial Cells
Interferon Receptors
Inflammation
Pattern Recognition Receptors
Toll-Like Receptors
Chronic Obstructive Pulmonary Disease
Serine
Cause of Death
Chronic Disease
Cell Death
Asthma
Cell Membrane
Cytokines
Delivery of Health Care
Membranes
Infection

Bibliographical note

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

Cite this

Stokes, C. A., Kaur, R., Edwards, M. R., Mondhe, M., Robinson, D., Prestwich, E. C., ... Parker, L. C. (2016). Human rhinovirus-induced inflammatory responses are inhibited by phosphatidylserine containing liposomes. 9(5), 1303-1316. https://doi.org/10.1038/mi.2015.137
Stokes, C.A. ; Kaur, R. ; Edwards, M.R. ; Mondhe, M. ; Robinson, D. ; Prestwich, E.C. ; Hume, R.D. ; Marshall, C.A. ; Perrie, Y. ; O'Donnell, V.B. ; Harwood, J.L. ; Sabroe, I. ; Parker, L.C. / Human rhinovirus-induced inflammatory responses are inhibited by phosphatidylserine containing liposomes. 2016 ; Vol. 9, No. 5. pp. 1303-1316.
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Stokes, CA, Kaur, R, Edwards, MR, Mondhe, M, Robinson, D, Prestwich, EC, Hume, RD, Marshall, CA, Perrie, Y, O'Donnell, VB, Harwood, JL, Sabroe, I & Parker, LC 2016, 'Human rhinovirus-induced inflammatory responses are inhibited by phosphatidylserine containing liposomes' vol. 9, no. 5, pp. 1303-1316. https://doi.org/10.1038/mi.2015.137

Human rhinovirus-induced inflammatory responses are inhibited by phosphatidylserine containing liposomes. / Stokes, C.A.; Kaur, R.; Edwards, M.R.; Mondhe, M.; Robinson, D.; Prestwich, E.C.; Hume, R.D.; Marshall, C.A.; Perrie, Y.; O'Donnell, V.B.; Harwood, J.L.; Sabroe, I.; Parker, L.C.

Vol. 9, No. 5, 09.2016, p. 1303-1316.

Research output: Contribution to journalArticle

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AU - O'Donnell, V.B.

AU - Harwood, J.L.

AU - Sabroe, I.

AU - Parker, L.C.

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Stokes CA, Kaur R, Edwards MR, Mondhe M, Robinson D, Prestwich EC et al. Human rhinovirus-induced inflammatory responses are inhibited by phosphatidylserine containing liposomes. 2016 Sep;9(5):1303-1316. https://doi.org/10.1038/mi.2015.137