Human single-donor composite skin substitutes based on collagen and polycaprolactone copolymer

Niann-Tzyy Dai, Ming-Kung Yeh, Chiao-Hsi Chiang, Ke-Chi Chen, Tsung-Hsun Liu, An-Chieh Feng, Li-Lien Chao, Chien-Ming Shih, Huey-Kang Sytwu, Shao-Liang Chen, Tim-Mo Chen, Eric F. Adams

Research output: Contribution to journalArticlepeer-review


The development and characterization of an enhanced composite skin substitute based on collagen and poly(e-caprolactone) are reported. Considering the features of excellent biocompatibility, easy-manipulated property and exempt from cross-linking related toxicity observed in the 1:20 biocomposites, skin substitutes were developed by seeding human single-donor keratinocytes and fibroblasts alone on both sides of the 1:20 biocomposite to allow for separation of two cell types and preserving cell signals transmission via micro-pores with a porosity of 28.8 ± 16.1 µm. The bi-layered skin substitute exhibited both differentiated epidermis and fibrous dermis in vitro. Less Keratinocyte Growth Factor production was measured in the co-cultured skin model compared to fibroblast alone condition indicating a favorable microenvironment for epidermal homeostasis. Moreover, fast wound closure, epidermal differentiation, and abundant dermal collagen deposition were observed in composite skin in vivo. In summary, the beneficial characteristics of the new skin substitutes exploited the potential for pharmaceutical screening and clinical application.
Original languageEnglish
Pages (from-to)21-25
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - 14 Aug 2009


  • skin substitute
  • collagen
  • poly(epsilon-caprolactone)
  • biocomposite
  • keratinocyte
  • fibroblast
  • porosity


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