Identification of a novel inositol phosphate recognition site: specific [3H]inositol hexakisphosphate binding to brain regions and cerebellar membranes

Phillip T. Hawkins, D.J.M. Reynolds, David R. Poyner, Michael R. Hanley

Research output: Contribution to journalArticle

Abstract

[3H]Inositol hexakisphosphate (InsP6) binds with a heterogeneous distribution to frozen sections of unfixed rat brain and is displaced by unlabelled InsP6. The pattern of binding correlates with binding to neuronal cell bodies. [3H]InsP6 binding to cerebellar membranes has been further characterised, is reversible, and saturable, and exhibits high specificity for inositol polyphosphates. The IC50 for competition by unlabelled InsP6 is approximately 100nM, whereas inositol 1,3,4,5,6 pentakisphosphate (Ins(13456)P5), inositol 1,3,4,5 tetrakisphosphate (Ins(1345)P4), and inositol 1,4,5 trisphosphate (Ins(145)P3) bind with an affinity at least one order of magnitude lower. [3H]InsP6 binding is clearly distinct from previously characterised Ins(145)P3 (ref. 1, 2) and Ins(1345)P4 (ref. 3) binding, both in terms of pharmacology and brain distribution.
Original languageEnglish
Pages (from-to)819-827
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume167
Issue number2
DOIs
Publication statusPublished - 16 Mar 1990

Keywords

  • animals
  • autoradiography
  • binding sites
  • brain
  • brain stem
  • cattle
  • cell membrane
  • cerebellum chromatography
  • high pressure liquid
  • inositol phosphates
  • kinetics
  • phytic acid
  • rats
  • tritium

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