Identification of Gnr1p, a negative regulator of Gα signalling in Schizosaccharomyces pombe, and its complementation by human Gβ subunits

Alan Goddard*, Graham Ladds, Rachel Forfar, John Davey

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

G protein-coupled receptors (GPCRs) are involved in the response of eukaryotic cells to a wide variety of stimuli, traditionally mediating their effects through heterotrimeric G proteins comprised of Gα, Gβ and Gγ subunits. The fission yeast Schizosaccharomyces pombe is an established tool for GPCR research, possessing two Gα-dependent signalling cascades. A complete Gαβγ complex has been characterised for the glucose-sensing pathway, but only the Gα subunit, Gpa1p, has been identified in the pheromone-response pathway. Here, we report the use of the yeast two-hybrid system to identify a novel protein, Gnr1p, which interacts with Gpa1p. Gnr1p is predicted to contain seven WD repeats and to adopt a structure similar to typical Gβ subunits. Disruption and overexpression studies reveal that Gnr1p negatively regulates the pheromone-response pathway but is not required for signalling. Human Gβ subunits complement the loss of Gnr1p, functioning as negative regulators of Gα signalling in fission yeast.

Original languageEnglish
Pages (from-to)840-851
Number of pages12
JournalFungal Genetics and Biology
Volume43
Issue number12
DOIs
Publication statusPublished - Dec 2006

Keywords

  • Gβ subunit
  • Gnr1p
  • Gpa1p
  • Pheromone
  • Schizosaccharomyces pombe
  • WD repeat protein

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