Identification of the HLA-DM/HLA-DR interface

Matthew N. Davies, Abigail Lamikanra, Clare E. Sansom, Darren R. Flower, David S. Moss, Paul J. Travers

Research output: Contribution to journalArticle

Abstract

Human leukocyte antigen (HLA)-DM is a critical participant in antigen presentation that catalyzes the dissociation of the Class II-associated Invariant chain-derived Peptide (CLIP) from the major histocompatibility complex (MHC) Class II molecules. There is competition amongst peptides for access to an MHC Class II groove and it has been hypothesised that DM functions as a 'peptide editor' that catalyzes the replacement of one peptide for another within the groove. It is established that the DM catalyst interacts directly with the MHC Class II but the precise location of the interface is unknown. Here, we combine previously described mutational data with molecular docking and energy minimisation simulations to identify a putative interaction site of >4000A2 which agrees with known point mutational data for both the DR and DM molecule. The docked structure is validated by comparison with experimental data and previously determined properties of protein-protein interfaces. A possible dissociation mechanism is suggested by the presence of an acidic cluster near the N terminus of the bound peptide.
Original languageEnglish
Pages (from-to)1063-1070
Number of pages8
JournalMolecular Immunology
Volume45
Issue number4
Early online date17 Sep 2007
DOIs
Publication statusPublished - Feb 2008

Keywords

  • MHC Class II
  • HLA-DR
  • HLA-DM
  • homology modelling
  • energy minimisation

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    Davies, M. N., Lamikanra, A., Sansom, C. E., Flower, D. R., Moss, D. S., & Travers, P. J. (2008). Identification of the HLA-DM/HLA-DR interface. Molecular Immunology, 45(4), 1063-1070. https://doi.org/10.1016/j.molimm.2007.07.033