Immunochemical detection of glyoxal DNA damage

Nalini Mistry, Mark D. Evans, Helen R. Griffiths, H. Kasai, Karl E. Herbert, Joseph Lunec

Research output: Contribution to journalArticle


The relevance of reactive oxygen species (ROS) in the pathogenesis of inflammatory diseases is widely documented. Immunochemical detection of ROS DNA adducts has been developed, however, recognition of glyoxal-DNA adducts has not previously been described. We have generated a polyclonal antibody that has shown increased antibody binding to ROS-modified DNA in comparison to native DNA. In addition, dose-dependent antibody binding to DNA modified with ascorbate alone was shown, with significant inhibition by desferrioxamine, catalase, and ethanol. Minimal inhibition was observed with uric acid, 1,10-phenanthroline and DMSO. However, antibody binding in the presence of EDTA increased 3500-fold. The involvement of hydrogen peroxide and hydroxyl radical in ascorbate-mediated DNA damage is consistent with ascorbate acting as a reducing agent for DNA-bound metal ions. Glyoxal is known to be formed during oxidation of ascorbate. Glyoxylated DNA, that previously had been proposed as a marker of oxidative damage, was recognised in a dose dependent manner using the antibody. We describe the potential use of our anti-ROS DNA antibody, that detects predominantly Fenton-type mediated damage to DNA and report on its specificity for the recognition of glyoxal-DNA adducts.
Original languageEnglish
Pages (from-to)1267-1273
Number of pages7
JournalFree Radical Biology and Medicine
Issue number9-10
Publication statusPublished - May 1999


  • DNA
  • glyoxal
  • antibodies
  • free radicals
  • immunochemical detection
  • ascorbic acid
  • fenton chemistry

Fingerprint Dive into the research topics of 'Immunochemical detection of glyoxal DNA damage'. Together they form a unique fingerprint.

  • Cite this

    Mistry, N., Evans, M. D., Griffiths, H. R., Kasai, H., Herbert, K. E., & Lunec, J. (1999). Immunochemical detection of glyoxal DNA damage. Free Radical Biology and Medicine, 26(9-10), 1267-1273.