Impact of Nanocapsules on Red Blood Cells Interplay Jointly Assessed by Optical Tweezers and Microscopy

Tatiana Avsievich; Yana Tarakanchikova; Ruixue Zhu; Alexey Popov; Alexander Bykov; Ilya Skovorodkin; Seppo Vainio; Igor Meglinski

doi:10.3390/mi11010019

Impact of Nanocapsules on Red Blood Cells Interplay Jointly Assessed by Optical Tweezers and Microscopy

Tatiana Avsievich, Yana Tarakanchikova, Ruixue Zhu, Alexey Popov, Alexander Bykov, Ilya Skovorodkin, Seppo Vainio, Igor Meglinski

Mechanical, Biomedical & Design Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

In the framework of novel medical paradigm the red blood cells (RBCs) have a great potential to be used as drug delivery carriers. This approach requires an ultimate understanding of the peculiarities of mutual interaction of RBC influenced by nano-materials composed the drugs. Optical tweezers (OT) is widely used to explore mechanisms of cells’ interaction with the ability to trap non-invasively, manipulate and displace living cells with a notably high accuracy. In the current study, the mutual interaction of RBC with polymeric nano-capsules (NCs) is investigated utilizing a two-channel OT system. The obtained results suggest that, in the presence of NCs, the RBC aggregation in plasma satisfies the ‘cross-bridges’ model. Complementarily, the allocation of NCs on the RBC membrane was observed by scanning electron microscopy (SEM), while for assessment of NCs-induced morphological changes the tests with the human mesenchymal stem cells (hMSC) was performed. The combined application of OT and advanced microscopy approaches brings new insights into the conception of direct observation of cells interaction influenced by NCs for the estimation of possible cytotoxic effects.

Original language	English
Journal	Micromachines
Volume	11
Issue number	1
DOIs	https://doi.org/10.3390/mi11010019
Publication status	Published - 23 Dec 2019

Bibliographical note

© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Funding: EDUFI Fellowship (TM-17-10370, TM-18-10820, T.A.), Oulun yliopiston apuraharahasto, No. 20180107, Suomen Kulttuurirahasto (grant No. 00190188, T.A.), CIMO Fellowship (TM-15-9729, Y.T.), EDUFI Fellowship (TM-17-10389, Y.T.), Academy of Finland (project no. 326126, Y.T.), China Scholarship Council (CSC No. 201706410089, R.Z.), Tauno Tönning Foundation (grant No. 20190104, R.Z.), Academy of Finland (grant No. 290596, 314369, 326126, A.B., A.P.).

Keywords

polymeric nanocapsules
red blood cells
optical tweezers
SEM; optical microscopy
cytotoxicity
mesenchymal stem cells

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10.3390/mi11010019

micromachines-11-00019
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Final published version, 6.81 MBLicence: CC BY 3.0

Cite this

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title = "Impact of Nanocapsules on Red Blood Cells Interplay Jointly Assessed by Optical Tweezers and Microscopy",

abstract = "In the framework of novel medical paradigm the red blood cells (RBCs) have a great potential to be used as drug delivery carriers. This approach requires an ultimate understanding of the peculiarities of mutual interaction of RBC influenced by nano-materials composed the drugs. Optical tweezers (OT) is widely used to explore mechanisms of cells{\textquoteright} interaction with the ability to trap non-invasively, manipulate and displace living cells with a notably high accuracy. In the current study, the mutual interaction of RBC with polymeric nano-capsules (NCs) is investigated utilizing a two-channel OT system. The obtained results suggest that, in the presence of NCs, the RBC aggregation in plasma satisfies the {\textquoteleft}cross-bridges{\textquoteright} model. Complementarily, the allocation of NCs on the RBC membrane was observed by scanning electron microscopy (SEM), while for assessment of NCs-induced morphological changes the tests with the human mesenchymal stem cells (hMSC) was performed. The combined application of OT and advanced microscopy approaches brings new insights into the conception of direct observation of cells interaction influenced by NCs for the estimation of possible cytotoxic effects.",

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author = "Tatiana Avsievich and Yana Tarakanchikova and Ruixue Zhu and Alexey Popov and Alexander Bykov and Ilya Skovorodkin and Seppo Vainio and Igor Meglinski",

note = "{\textcopyright} 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Funding: EDUFI Fellowship (TM-17-10370, TM-18-10820, T.A.), Oulun yliopiston apuraharahasto, No. 20180107, Suomen Kulttuurirahasto (grant No. 00190188, T.A.), CIMO Fellowship (TM-15-9729, Y.T.), EDUFI Fellowship (TM-17-10389, Y.T.), Academy of Finland (project no. 326126, Y.T.), China Scholarship Council (CSC No. 201706410089, R.Z.), Tauno T{\"o}nning Foundation (grant No. 20190104, R.Z.), Academy of Finland (grant No. 290596, 314369, 326126, A.B., A.P.). ",

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AU - Avsievich, Tatiana

AU - Tarakanchikova, Yana

AU - Zhu, Ruixue

AU - Popov, Alexey

AU - Bykov, Alexander

AU - Skovorodkin, Ilya

AU - Vainio, Seppo

AU - Meglinski, Igor

N1 - © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Funding: EDUFI Fellowship (TM-17-10370, TM-18-10820, T.A.), Oulun yliopiston apuraharahasto, No. 20180107, Suomen Kulttuurirahasto (grant No. 00190188, T.A.), CIMO Fellowship (TM-15-9729, Y.T.), EDUFI Fellowship (TM-17-10389, Y.T.), Academy of Finland (project no. 326126, Y.T.), China Scholarship Council (CSC No. 201706410089, R.Z.), Tauno Tönning Foundation (grant No. 20190104, R.Z.), Academy of Finland (grant No. 290596, 314369, 326126, A.B., A.P.).

PY - 2019/12/23

Y1 - 2019/12/23

N2 - In the framework of novel medical paradigm the red blood cells (RBCs) have a great potential to be used as drug delivery carriers. This approach requires an ultimate understanding of the peculiarities of mutual interaction of RBC influenced by nano-materials composed the drugs. Optical tweezers (OT) is widely used to explore mechanisms of cells’ interaction with the ability to trap non-invasively, manipulate and displace living cells with a notably high accuracy. In the current study, the mutual interaction of RBC with polymeric nano-capsules (NCs) is investigated utilizing a two-channel OT system. The obtained results suggest that, in the presence of NCs, the RBC aggregation in plasma satisfies the ‘cross-bridges’ model. Complementarily, the allocation of NCs on the RBC membrane was observed by scanning electron microscopy (SEM), while for assessment of NCs-induced morphological changes the tests with the human mesenchymal stem cells (hMSC) was performed. The combined application of OT and advanced microscopy approaches brings new insights into the conception of direct observation of cells interaction influenced by NCs for the estimation of possible cytotoxic effects.

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KW - optical tweezers

KW - SEM; optical microscopy

KW - cytotoxicity

KW - mesenchymal stem cells

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DO - 10.3390/mi11010019

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SN - 2072-666X

VL - 11

JO - Micromachines

JF - Micromachines

IS - 1

ER -

Impact of Nanocapsules on Red Blood Cells Interplay Jointly Assessed by Optical Tweezers and Microscopy

Abstract

Bibliographical note

Keywords

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