Impact of Plasmonic Nanoparticles on Poikilocytosis and Microrheological Properties of Erythrocytes

Tatiana Avsievich; Ruixue Zhu; Alexey P. Popov; Alexander Yatskovskiy; Anton A. Popov; Gleb Tikhonowsky; Andrei I. Pastukhov; Sergei Klimentov; Alexander Bykov; Andrei Kabashin; Igor Meglinski

doi:10.3390/pharmaceutics15041046

Impact of Plasmonic Nanoparticles on Poikilocytosis and Microrheological Properties of Erythrocytes

Tatiana Avsievich
, Ruixue Zhu
, Alexey P. Popov
, Alexander Yatskovskiy
, Anton A. Popov
, Gleb Tikhonowsky
, Andrei I. Pastukhov
, Sergei Klimentov
, Alexander Bykov
, Andrei Kabashin
, Igor Meglinski

Optoelectronics and Measurement Techniques, University of Oulu, 90570 Oulu, Finland.
VTT Technical Research Centre of Finland, Kaitovayla 1, 90590 Oulu, Finland
Department of Histology, Cytology and Embryology, Institute of Clinical Medicine N.V. Sklifosovsky, I.M. Sechenov First Moscow State Medical University, Trubetskaya Street 8, 119991 Moscow, Russia
Institute of Engineering Physics for Biomedicine (PhysBio), National Research Nuclear University (MEPhI), Kashirskoe Shosse, 31, 115409 Moscow, Russia
CNRS, LP3, Aix-Marseille University, 163 Av. de Luminy, 13009 Marseille, France

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

11 Downloads (Pure)

Abstract

Plasmonic nanoparticles (NP) possess great potential in photothermal therapy and diagnostics. However, novel NP require a detailed examination for potential toxicity and peculiarities of interaction with cells. Red blood cells (RBC) are important for NP distribution and the development of hybrid RBC-NP delivery systems. This research explored RBC alterations induced by noble (Au and Ag) and nitride-based (TiN and ZrN) laser-synthesized plasmonic NP. Optical tweezers and conventional microscopy modalities indicated the effects arising at non-hemolytic levels, such as RBC poikilocytosis, and alterations in RBC microrheological parameters, elasticity and intercellular interactions. Aggregation and deformability significantly decreased for echinocytes independently of NP type, while for intact RBC, all NP except Ag NP increased the interaction forces but had no effect on RBC deformability. RBC poikilocytosis promoted by NP at concentration 50 μg mL−1 was more pronounced for Au and Ag NP, compared to TiN and ZrN NP. Nitride-based NP demonstrated better biocompatibility towards RBC and higher photothermal efficiency than their noble metal counterparts.

Original language	English
Article number	1046
Number of pages	15
Journal	Pharmaceutics
Volume	15
Issue number	4
Early online date	23 Mar 2023
DOIs	https://doi.org/10.3390/pharmaceutics15041046
Publication status	Published - Apr 2023

Bibliographical note

Copyright © 2023 by the authors.
Licensee MDPI, Basel, Switzerland.
This article is an open access article
distributed under the terms and
conditions of the Creative Commons
Attribution (CC BY) license (https://
creativecommons.org/licenses/by/
4.0/).

Funding Information:
Current study partially supported by the Suomen Kulttuurirahasto (grant No. 00190188), China Scholarship Council (CSC No. 201706410089) and the INFOTECH and the Academy of Finland (project 325097). The authors also acknowledge the contribution of Russian Science Foundation (Project: 19-72-30012 and Project: 22-65-00096). I.M. acknowledges the support from the Leverhulme Trust and The Royal Society (Ref. no.: APX\R1\211232 APEX Awards 2021).

Publisher Copyright:
© 2023 by the authors.

Keywords

aggregation
cell–cell interactions
microrheology
microscopy
optical manipulation
plasmonic nanoparticles
poikilocytosis
red blood cells

Access to Document

10.3390/pharmaceutics15041046Licence: CC BY 4.0

Avsievichetal_2023_VoR
Copyright © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
Final published version, 78 MBLicence: CC BY 4.0

Cite this

@article{23fe0e7d18774711bacb2853eb3b8bf1,

title = "Impact of Plasmonic Nanoparticles on Poikilocytosis and Microrheological Properties of Erythrocytes",

abstract = "Plasmonic nanoparticles (NP) possess great potential in photothermal therapy and diagnostics. However, novel NP require a detailed examination for potential toxicity and peculiarities of interaction with cells. Red blood cells (RBC) are important for NP distribution and the development of hybrid RBC-NP delivery systems. This research explored RBC alterations induced by noble (Au and Ag) and nitride-based (TiN and ZrN) laser-synthesized plasmonic NP. Optical tweezers and conventional microscopy modalities indicated the effects arising at non-hemolytic levels, such as RBC poikilocytosis, and alterations in RBC microrheological parameters, elasticity and intercellular interactions. Aggregation and deformability significantly decreased for echinocytes independently of NP type, while for intact RBC, all NP except Ag NP increased the interaction forces but had no effect on RBC deformability. RBC poikilocytosis promoted by NP at concentration 50 μg mL−1 was more pronounced for Au and Ag NP, compared to TiN and ZrN NP. Nitride-based NP demonstrated better biocompatibility towards RBC and higher photothermal efficiency than their noble metal counterparts.",

keywords = "aggregation, cell–cell interactions, microrheology, microscopy, optical manipulation, plasmonic nanoparticles, poikilocytosis, red blood cells",

author = "Tatiana Avsievich and Ruixue Zhu and Popov, \{Alexey P.\} and Alexander Yatskovskiy and Popov, \{Anton A.\} and Gleb Tikhonowsky and Pastukhov, \{Andrei I.\} and Sergei Klimentov and Alexander Bykov and Andrei Kabashin and Igor Meglinski",

note = "Copyright {\textcopyright} 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). Funding Information: Current study partially supported by the Suomen Kulttuurirahasto (grant No. 00190188), China Scholarship Council (CSC No. 201706410089) and the INFOTECH and the Academy of Finland (project 325097). The authors also acknowledge the contribution of Russian Science Foundation (Project: 19-72-30012 and Project: 22-65-00096). I.M. acknowledges the support from the Leverhulme Trust and The Royal Society (Ref. no.: APX\textbackslash{}R1\textbackslash{}211232 APEX Awards 2021). Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

month = apr,

doi = "10.3390/pharmaceutics15041046",

language = "English",

volume = "15",

journal = "Pharmaceutics",

issn = "1999-4923",

publisher = "MDPI AG",

number = "4",

}

TY - JOUR

T1 - Impact of Plasmonic Nanoparticles on Poikilocytosis and Microrheological Properties of Erythrocytes

AU - Avsievich, Tatiana

AU - Zhu, Ruixue

AU - Popov, Alexey P.

AU - Yatskovskiy, Alexander

AU - Popov, Anton A.

AU - Tikhonowsky, Gleb

AU - Pastukhov, Andrei I.

AU - Klimentov, Sergei

AU - Bykov, Alexander

AU - Kabashin, Andrei

AU - Meglinski, Igor

N1 - Copyright © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). Funding Information: Current study partially supported by the Suomen Kulttuurirahasto (grant No. 00190188), China Scholarship Council (CSC No. 201706410089) and the INFOTECH and the Academy of Finland (project 325097). The authors also acknowledge the contribution of Russian Science Foundation (Project: 19-72-30012 and Project: 22-65-00096). I.M. acknowledges the support from the Leverhulme Trust and The Royal Society (Ref. no.: APX\R1\211232 APEX Awards 2021). Publisher Copyright: © 2023 by the authors.

PY - 2023/4

Y1 - 2023/4

N2 - Plasmonic nanoparticles (NP) possess great potential in photothermal therapy and diagnostics. However, novel NP require a detailed examination for potential toxicity and peculiarities of interaction with cells. Red blood cells (RBC) are important for NP distribution and the development of hybrid RBC-NP delivery systems. This research explored RBC alterations induced by noble (Au and Ag) and nitride-based (TiN and ZrN) laser-synthesized plasmonic NP. Optical tweezers and conventional microscopy modalities indicated the effects arising at non-hemolytic levels, such as RBC poikilocytosis, and alterations in RBC microrheological parameters, elasticity and intercellular interactions. Aggregation and deformability significantly decreased for echinocytes independently of NP type, while for intact RBC, all NP except Ag NP increased the interaction forces but had no effect on RBC deformability. RBC poikilocytosis promoted by NP at concentration 50 μg mL−1 was more pronounced for Au and Ag NP, compared to TiN and ZrN NP. Nitride-based NP demonstrated better biocompatibility towards RBC and higher photothermal efficiency than their noble metal counterparts.

AB - Plasmonic nanoparticles (NP) possess great potential in photothermal therapy and diagnostics. However, novel NP require a detailed examination for potential toxicity and peculiarities of interaction with cells. Red blood cells (RBC) are important for NP distribution and the development of hybrid RBC-NP delivery systems. This research explored RBC alterations induced by noble (Au and Ag) and nitride-based (TiN and ZrN) laser-synthesized plasmonic NP. Optical tweezers and conventional microscopy modalities indicated the effects arising at non-hemolytic levels, such as RBC poikilocytosis, and alterations in RBC microrheological parameters, elasticity and intercellular interactions. Aggregation and deformability significantly decreased for echinocytes independently of NP type, while for intact RBC, all NP except Ag NP increased the interaction forces but had no effect on RBC deformability. RBC poikilocytosis promoted by NP at concentration 50 μg mL−1 was more pronounced for Au and Ag NP, compared to TiN and ZrN NP. Nitride-based NP demonstrated better biocompatibility towards RBC and higher photothermal efficiency than their noble metal counterparts.

KW - aggregation

KW - cell–cell interactions

KW - microrheology

KW - microscopy

KW - optical manipulation

KW - plasmonic nanoparticles

KW - poikilocytosis

KW - red blood cells

UR - https://www.mdpi.com/1999-4923/15/4/1046

UR - https://www.scopus.com/pages/publications/85154595842

U2 - 10.3390/pharmaceutics15041046

DO - 10.3390/pharmaceutics15041046

M3 - Article

C2 - 37111532

SN - 1999-4923

VL - 15

JO - Pharmaceutics

JF - Pharmaceutics

IS - 4

M1 - 1046

ER -

Impact of Plasmonic Nanoparticles on Poikilocytosis and Microrheological Properties of Erythrocytes

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this