Impairments of cerebral blood flow microcirculation in rats brought on by cardiac cessation and respiratory arrest

Gennadii Piavchenko; Igor Kozlov; Viktor Dremin; Dmitry Stavtsev; Evgeniya Seryogina; Ksenia Kandurova; Valery Shupletsov; Konstantin Lapin; Alexander Alekseyev; Sergey Kuznetsov; Alexander Bykov; Andrey Dunaev; Igor Meglinski

doi:10.1002/jbio.202100216

Impairments of cerebral blood flow microcirculation in rats brought on by cardiac cessation and respiratory arrest

Gennadii Piavchenko^*, Igor Kozlov, Viktor Dremin, Dmitry Stavtsev, Evgeniya Seryogina, Ksenia Kandurova, Valery Shupletsov, Konstantin Lapin, Alexander Alekseyev, Sergey Kuznetsov, Alexander Bykov, Andrey Dunaev, Igor Meglinski

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The impairments of cerebral blood flow microcirculation brought on by cardiac and respiratory arrest were assessed with multi-modal diagnostic facilities, utilising laser speckle contrast imaging, fluorescence spectroscopy and diffuse reflectance spectroscopy. The results of laser speckle contrast imaging show a notable reduction of cerebral blood flow in small and medium size vessels during a few minutes of respiratory arrest, while the same effect was observed in large sinuses and their branches during the circulatory cessation. Concurrently, the redox ratio assessed with fluorescence spectroscopy indicates progressing hypoxia, NADH accumulation and increase of FAD consumption. The results of diffuse reflectance spectra measurements display a more rapid grow of the perfusion of deoxygenated blood in case of circulatory impairment. In addition, consequent histopathological analysis performed by using new tissue staining procedure developed in-house. It shows notably higher reduction of size of the neurons due to their wrinkling within brain tissues influenced by circulation impair. Whereas, the brain tissues altered with the respiratory arrest demonstrate focal perivascular oedema and mild hypoxic changes of neuronal morphology. Thus, the study suggests that consequences of a cessation of cerebral blood flow become more dramatic and dangerous compare to respiratory arrest.

Original language	English
Article number	e202100216
Journal	Journal of Biophotonics
Volume	14
Issue number	12
Early online date	17 Sept 2021
DOIs	https://doi.org/10.1002/jbio.202100216
Publication status	Published - Dec 2021

Bibliographical note

© 2021 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Funding: The authors disclose receipt of ﬁnancial support for the research, authorship, and/or publication of this article as fol-lowing: G.P. and S.K. acknowledge the support from the Ministry of Science and Higher Education of the Russian Federation withing the framework of State support for the creation and development of World-Class research Centres “Digital Biodesign and Personalized Healthcare” No 075-15-2020-926.V.D. kindly acknowledges personal support from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No. 839888.A.D. and I.M. acknowledge funding from the Academy of Fin-land (grant No. 326204). This work has been also partially supported by the European Union’s Horizon 2020 research and innovation programme under grant agreement No.863214- NEUROPA project.

Keywords

brain imaging
cardiac cessation
cerebral blood flow
laser speckle contrast
microcirculation
respiratory arrest

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VoR- Impairments of cerebral blood flow microcirculation in rats
© 2021 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Final published version, 4.93 MBLicence: CC BY-NC-ND 3.0

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Piavchenko, G., Kozlov, I., Dremin, V., Stavtsev, D., Seryogina, E., Kandurova, K., Shupletsov, V., Lapin, K., Alekseyev, A., Kuznetsov, S., Bykov, A., Dunaev, A., & Meglinski, I. (2021). Impairments of cerebral blood flow microcirculation in rats brought on by cardiac cessation and respiratory arrest. Journal of Biophotonics, 14(12), Article e202100216. https://doi.org/10.1002/jbio.202100216

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abstract = "The impairments of cerebral blood flow microcirculation brought on by cardiac and respiratory arrest were assessed with multi-modal diagnostic facilities, utilising laser speckle contrast imaging, fluorescence spectroscopy and diffuse reflectance spectroscopy. The results of laser speckle contrast imaging show a notable reduction of cerebral blood flow in small and medium size vessels during a few minutes of respiratory arrest, while the same effect was observed in large sinuses and their branches during the circulatory cessation. Concurrently, the redox ratio assessed with fluorescence spectroscopy indicates progressing hypoxia, NADH accumulation and increase of FAD consumption. The results of diffuse reflectance spectra measurements display a more rapid grow of the perfusion of deoxygenated blood in case of circulatory impairment. In addition, consequent histopathological analysis performed by using new tissue staining procedure developed in-house. It shows notably higher reduction of size of the neurons due to their wrinkling within brain tissues influenced by circulation impair. Whereas, the brain tissues altered with the respiratory arrest demonstrate focal perivascular oedema and mild hypoxic changes of neuronal morphology. Thus, the study suggests that consequences of a cessation of cerebral blood flow become more dramatic and dangerous compare to respiratory arrest.",

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author = "Gennadii Piavchenko and Igor Kozlov and Viktor Dremin and Dmitry Stavtsev and Evgeniya Seryogina and Ksenia Kandurova and Valery Shupletsov and Konstantin Lapin and Alexander Alekseyev and Sergey Kuznetsov and Alexander Bykov and Andrey Dunaev and Igor Meglinski",

note = "{\textcopyright} 2021 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Funding: The authors disclose receipt of ﬁnancial support for the research, authorship, and/or publication of this article as fol-lowing: G.P. and S.K. acknowledge the support from the Ministry of Science and Higher Education of the Russian Federation withing the framework of State support for the creation and development of World-Class research Centres “Digital Biodesign and Personalized Healthcare” No 075-15-2020-926.V.D. kindly acknowledges personal support from the European Union{\textquoteright}s Horizon 2020 research and innovation program under the Marie Sk{\l}odowska-Curie grant agreement No. 839888.A.D. and I.M. acknowledge funding from the Academy of Fin-land (grant No. 326204). This work has been also partially supported by the European Union{\textquoteright}s Horizon 2020 research and innovation programme under grant agreement No.863214- NEUROPA project.",

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Piavchenko, G, Kozlov, I, Dremin, V, Stavtsev, D, Seryogina, E, Kandurova, K, Shupletsov, V, Lapin, K, Alekseyev, A, Kuznetsov, S, Bykov, A, Dunaev, A & Meglinski, I 2021, 'Impairments of cerebral blood flow microcirculation in rats brought on by cardiac cessation and respiratory arrest', Journal of Biophotonics, vol. 14, no. 12, e202100216. https://doi.org/10.1002/jbio.202100216

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T1 - Impairments of cerebral blood flow microcirculation in rats brought on by cardiac cessation and respiratory arrest

AU - Piavchenko, Gennadii

AU - Kozlov, Igor

AU - Dremin, Viktor

AU - Stavtsev, Dmitry

AU - Seryogina, Evgeniya

AU - Kandurova, Ksenia

AU - Shupletsov, Valery

AU - Lapin, Konstantin

AU - Alekseyev, Alexander

AU - Kuznetsov, Sergey

AU - Bykov, Alexander

AU - Dunaev, Andrey

AU - Meglinski, Igor

N1 - © 2021 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Funding: The authors disclose receipt of ﬁnancial support for the research, authorship, and/or publication of this article as fol-lowing: G.P. and S.K. acknowledge the support from the Ministry of Science and Higher Education of the Russian Federation withing the framework of State support for the creation and development of World-Class research Centres “Digital Biodesign and Personalized Healthcare” No 075-15-2020-926.V.D. kindly acknowledges personal support from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No. 839888.A.D. and I.M. acknowledge funding from the Academy of Fin-land (grant No. 326204). This work has been also partially supported by the European Union’s Horizon 2020 research and innovation programme under grant agreement No.863214- NEUROPA project.

PY - 2021/12

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N2 - The impairments of cerebral blood flow microcirculation brought on by cardiac and respiratory arrest were assessed with multi-modal diagnostic facilities, utilising laser speckle contrast imaging, fluorescence spectroscopy and diffuse reflectance spectroscopy. The results of laser speckle contrast imaging show a notable reduction of cerebral blood flow in small and medium size vessels during a few minutes of respiratory arrest, while the same effect was observed in large sinuses and their branches during the circulatory cessation. Concurrently, the redox ratio assessed with fluorescence spectroscopy indicates progressing hypoxia, NADH accumulation and increase of FAD consumption. The results of diffuse reflectance spectra measurements display a more rapid grow of the perfusion of deoxygenated blood in case of circulatory impairment. In addition, consequent histopathological analysis performed by using new tissue staining procedure developed in-house. It shows notably higher reduction of size of the neurons due to their wrinkling within brain tissues influenced by circulation impair. Whereas, the brain tissues altered with the respiratory arrest demonstrate focal perivascular oedema and mild hypoxic changes of neuronal morphology. Thus, the study suggests that consequences of a cessation of cerebral blood flow become more dramatic and dangerous compare to respiratory arrest.

AB - The impairments of cerebral blood flow microcirculation brought on by cardiac and respiratory arrest were assessed with multi-modal diagnostic facilities, utilising laser speckle contrast imaging, fluorescence spectroscopy and diffuse reflectance spectroscopy. The results of laser speckle contrast imaging show a notable reduction of cerebral blood flow in small and medium size vessels during a few minutes of respiratory arrest, while the same effect was observed in large sinuses and their branches during the circulatory cessation. Concurrently, the redox ratio assessed with fluorescence spectroscopy indicates progressing hypoxia, NADH accumulation and increase of FAD consumption. The results of diffuse reflectance spectra measurements display a more rapid grow of the perfusion of deoxygenated blood in case of circulatory impairment. In addition, consequent histopathological analysis performed by using new tissue staining procedure developed in-house. It shows notably higher reduction of size of the neurons due to their wrinkling within brain tissues influenced by circulation impair. Whereas, the brain tissues altered with the respiratory arrest demonstrate focal perivascular oedema and mild hypoxic changes of neuronal morphology. Thus, the study suggests that consequences of a cessation of cerebral blood flow become more dramatic and dangerous compare to respiratory arrest.

KW - brain imaging

KW - cardiac cessation

KW - cerebral blood flow

KW - laser speckle contrast

KW - microcirculation

KW - respiratory arrest

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DO - 10.1002/jbio.202100216

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M1 - e202100216

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Impairments of cerebral blood flow microcirculation in rats brought on by cardiac cessation and respiratory arrest

Abstract

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Keywords

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