In silico identification of novel G protein coupled receptors

Matthew N. Davies, Darren R. Flower

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Abstract

G-protein coupled receptors (GPCRs) are a superfamily of membrane integral proteins responsible for a large number of physiological functions. Approximately 50% of marketed drugs are targeted toward a GPCR. Despite showing a high degree of structural homology, there is a large variance in sequence within the GPCR superfamily which has lead to difficulties in identifying and classifying potential new GPCR proteins. Here the various computational techniques that can be used to characterize a novel GPCR protein are discussed, including both alignment-based and alignment-free approaches. In addition, the application of homology modeling to building the three-dimensional structures of GPCRs is described.
Original languageEnglish
Title of host publicationMembrane proteomics
Subtitle of host publicationmethods and protocols
EditorsMatthew J. Peirce, Robin Wait
PublisherHumana Press
Pages25-36
Number of pages12
ISBN (Electronic)978-1-60327-310-7
ISBN (Print)978-1-60327-309-1
DOIs
Publication statusPublished - 2009

Publication series

NameMethods in molecular biology
PublisherSpringer
Volume528
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Fingerprint

G-Protein-Coupled Receptors
Computer Simulation
Membrane Proteins
Proteins
Pharmaceutical Preparations

Cite this

Davies, M. N., & Flower, D. R. (2009). In silico identification of novel G protein coupled receptors. In M. J. Peirce, & R. Wait (Eds.), Membrane proteomics: methods and protocols (pp. 25-36). (Methods in molecular biology; Vol. 528). Humana Press. https://doi.org/10.1007/978-1-60327-310-7_2
Davies, Matthew N. ; Flower, Darren R. / In silico identification of novel G protein coupled receptors. Membrane proteomics: methods and protocols. editor / Matthew J. Peirce ; Robin Wait. Humana Press, 2009. pp. 25-36 (Methods in molecular biology).
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Davies, MN & Flower, DR 2009, In silico identification of novel G protein coupled receptors. in MJ Peirce & R Wait (eds), Membrane proteomics: methods and protocols. Methods in molecular biology, vol. 528, Humana Press, pp. 25-36. https://doi.org/10.1007/978-1-60327-310-7_2

In silico identification of novel G protein coupled receptors. / Davies, Matthew N.; Flower, Darren R.

Membrane proteomics: methods and protocols. ed. / Matthew J. Peirce; Robin Wait. Humana Press, 2009. p. 25-36 (Methods in molecular biology; Vol. 528).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

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Davies MN, Flower DR. In silico identification of novel G protein coupled receptors. In Peirce MJ, Wait R, editors, Membrane proteomics: methods and protocols. Humana Press. 2009. p. 25-36. (Methods in molecular biology). https://doi.org/10.1007/978-1-60327-310-7_2