G-protein coupled receptors (GPCRs) are a superfamily of membrane integral proteins responsible for a large number of physiological functions. Approximately 50% of marketed drugs are targeted toward a GPCR. Despite showing a high degree of structural homology, there is a large variance in sequence within the GPCR superfamily which has lead to difficulties in identifying and classifying potential new GPCR proteins. Here the various computational techniques that can be used to characterize a novel GPCR protein are discussed, including both alignment-based and alignment-free approaches. In addition, the application of homology modeling to building the three-dimensional structures of GPCRs is described.
|Title of host publication||Membrane proteomics|
|Subtitle of host publication||methods and protocols|
|Editors||Matthew J. Peirce, Robin Wait|
|Number of pages||12|
|Publication status||Published - 2009|
|Name||Methods in molecular biology|
Davies, M. N., & Flower, D. R. (2009). In silico identification of novel G protein coupled receptors. In M. J. Peirce, & R. Wait (Eds.), Membrane proteomics: methods and protocols (pp. 25-36). (Methods in molecular biology; Vol. 528). Humana Press. https://doi.org/10.1007/978-1-60327-310-7_2