In vitro chronic neurotoxicity assays: Present and future developments

D. A. Nagel, E. J. Hill, E. K. Woehrling, M. D. Coleman

Research output: Chapter in Book/Published conference outputChapter


Pressure from our aging population’s vulnerability to neurodegenerative disease necessitates the development of experimental platforms to research brain pathology and new drug design, as well as the detection of toxic impact on both the adult and developing CNS. Although exciting human stem cell models are under development, the NT2.D1 teratocarcinoma model differentiates into a co-culture of neurones and astrocytes which, combined with bioreactors and models of the human blood brain barrier such as hCMEC/D3 cells, affords probably the most relevant and flexible opportunity available to investigate teratogenic and toxic chemical impact on the most basic functional and structural aspects of the human CNS.

Original languageEnglish
Title of host publicationComprehensive Biotechnology
Number of pages15
ISBN (Electronic)9780444640475
ISBN (Print)9780444640468
Publication statusPublished - 1 Jan 2019


  • Astrocyte
  • Bioreactor
  • Blood-brain barrier
  • Co-culture
  • Differentiation
  • hCMEC/D3
  • Human
  • Neuron
  • NT2.D1
  • Stem cell
  • Teratogenicity


Dive into the research topics of 'In vitro chronic neurotoxicity assays: Present and future developments'. Together they form a unique fingerprint.

Cite this