In vitro influence of 520 nm diode laser irradiation on red blood cell spontaneous aggregation studied by optical tweezers and light microscopy

Ruixue Zhu; Tatiana Avsievich; Alexey Popov; Alexander Bykov; Igor Meglinski

doi:10.1117/12.2595118

In vitro influence of 520 nm diode laser irradiation on red blood cell spontaneous aggregation studied by optical tweezers and light microscopy

Ruixue Zhu^*, Tatiana Avsievich, Alexey Popov, Alexander Bykov, Igor Meglinski

^*Corresponding author for this work

Research output: Contribution to journal › Conference article › peer-review

Abstract

The laser has biomodulation effects on blood properties thus could regulate the microcirculation. The laser-RBC interaction mechanism is unclear, whereas most studies provide statistical results and lack detailed observations of laser irradiation effects on blood rheology. This study is designated to probe the in vitro effects of 520 nm diode laser irradiation on red blood cells (RBCs) mutual interaction properties in spontaneous aggregation process in autologous plasma by optical tweezers (OTs), with an attempt to reveal the laser-RBC interaction outcomes at a single-cell level. The results preliminarily show that though the laser irradiation statistically inhibited the increase of the size of RBC aggregates compared with the non-irradiating group, the aggregation force between single RBCs increased slightly with the time of irradiation.

Original language	English
Article number	1179809
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	11798
DOIs	https://doi.org/10.1117/12.2595118
Publication status	Published - 1 Aug 2021
Event	Optical Trapping and Optical Micromanipulation XVIII 2021 - San Diego, United States Duration: 1 Aug 2021 → 5 Aug 2021

Bibliographical note

Copyright 2021 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

Funding Information:
This work was partially funded by the China Scholarship Council (CSC No. 201706410089, R.Z.). R.Z. acknowledges X.Y. Su (School of Science, Beijing Jiaotong University, Beijing, China) for technical help in laser irradiation source selection. The authors also acknowledge the contribution of Russian Science Foundation (Projects: 19-72-30012 and 20-64-46003). I.M. acknowledges partial support from the Academy of Finland (project 325,097).

Keywords

laser irradiation
light microscopy
optical tweezers
RBC aggregation
Red blood cells (RBCs)

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10.1117/12.2595118

In vitro influence of 520 nm diode laser irradiation
Copyright 2021 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Final published version, 4.07 MB

Cite this

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title = "In vitro influence of 520 nm diode laser irradiation on red blood cell spontaneous aggregation studied by optical tweezers and light microscopy",

abstract = "The laser has biomodulation effects on blood properties thus could regulate the microcirculation. The laser-RBC interaction mechanism is unclear, whereas most studies provide statistical results and lack detailed observations of laser irradiation effects on blood rheology. This study is designated to probe the in vitro effects of 520 nm diode laser irradiation on red blood cells (RBCs) mutual interaction properties in spontaneous aggregation process in autologous plasma by optical tweezers (OTs), with an attempt to reveal the laser-RBC interaction outcomes at a single-cell level. The results preliminarily show that though the laser irradiation statistically inhibited the increase of the size of RBC aggregates compared with the non-irradiating group, the aggregation force between single RBCs increased slightly with the time of irradiation. ",

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In vitro influence of 520 nm diode laser irradiation on red blood cell spontaneous aggregation studied by optical tweezers and light microscopy. / Zhu, Ruixue; Avsievich, Tatiana; Popov, Alexey et al.
In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 11798, 1179809, 01.08.2021.

Research output: Contribution to journal › Conference article › peer-review

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AU - Meglinski, Igor

N1 - Copyright 2021 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. Funding Information: This work was partially funded by the China Scholarship Council (CSC No. 201706410089, R.Z.). R.Z. acknowledges X.Y. Su (School of Science, Beijing Jiaotong University, Beijing, China) for technical help in laser irradiation source selection. The authors also acknowledge the contribution of Russian Science Foundation (Projects: 19-72-30012 and 20-64-46003). I.M. acknowledges partial support from the Academy of Finland (project 325,097).

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N2 - The laser has biomodulation effects on blood properties thus could regulate the microcirculation. The laser-RBC interaction mechanism is unclear, whereas most studies provide statistical results and lack detailed observations of laser irradiation effects on blood rheology. This study is designated to probe the in vitro effects of 520 nm diode laser irradiation on red blood cells (RBCs) mutual interaction properties in spontaneous aggregation process in autologous plasma by optical tweezers (OTs), with an attempt to reveal the laser-RBC interaction outcomes at a single-cell level. The results preliminarily show that though the laser irradiation statistically inhibited the increase of the size of RBC aggregates compared with the non-irradiating group, the aggregation force between single RBCs increased slightly with the time of irradiation.

AB - The laser has biomodulation effects on blood properties thus could regulate the microcirculation. The laser-RBC interaction mechanism is unclear, whereas most studies provide statistical results and lack detailed observations of laser irradiation effects on blood rheology. This study is designated to probe the in vitro effects of 520 nm diode laser irradiation on red blood cells (RBCs) mutual interaction properties in spontaneous aggregation process in autologous plasma by optical tweezers (OTs), with an attempt to reveal the laser-RBC interaction outcomes at a single-cell level. The results preliminarily show that though the laser irradiation statistically inhibited the increase of the size of RBC aggregates compared with the non-irradiating group, the aggregation force between single RBCs increased slightly with the time of irradiation.

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In vitro influence of 520 nm diode laser irradiation on red blood cell spontaneous aggregation studied by optical tweezers and light microscopy

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