In vivo effects of tailored laminin-332 α3 conjugated scaffolds enhances wound healing: a histomorphometric analysis

Gopinath Damodaran, William H.C. Tiong, Russell Collighan, Harshad Navsaria, Martin Griffin, Abhay Pandit

Research output: Contribution to journalArticle

Abstract

Surface modification techniques have been used to develop biomimetic scaffolds by incorporating cell adhesion peptides. In our previous work, we have shown the tethering of laminin-332 α3 chain to type I collagen scaffold using microbial transglutaminase (mTGase), promotes cell adhesion, migration, and proliferation. In this study, we evaluated the wound healing properties of tailored laminin-332 α3 chain (peptide A: PPFLMLLKGSTR) tethered to a type I collagen scaffold using mTGase by incorporating transglutaminase substrate peptide sequences containing either glutamine (peptide B: PPFLMLLKGSTREAQQIVM) or lysine (peptide C: PPFLMLLKGSTRKKKKG) in rat full-thickness wound model at two different time points (7 and 21 days). Histological evaluations were assessed for wound closure, epithelialization, angiogenesis, inflammatory, fibroblastic cellular infiltrations, and quantified using stereological methods (p < 0.05). Peptide A and B tethered to collagen scaffold using mTGase stimulated neovascularization, decreased the inflammatory cell infiltration and prominently enhanced the fibroblast proliferation which significantly accelerated the wound healing process. We conclude that surface modification by incorporating motif of laminin-332 α3 chain (peptide A: PPFLMLLK GSTR) domain and transglutaminase substrate to the laminin-332 α3 chain (peptide B: PPFLMLLKGSTREAQQIVM) using mTGase may be a potential candidate for tissue engineering applications and skin regeneration. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A:2788-2795, 2013.

LanguageEnglish
Pages2788-2795
Number of pages8
JournalJournal of Biomedical Materials Research: Part A
Volume101
Issue number10
DOIs
Publication statusE-pub ahead of print - 5 Mar 2013

Fingerprint

Transglutaminases
Scaffolds
Peptides
Scaffolds (biology)
Cell adhesion
Collagen
Collagen Type I
Infiltration
Surface treatment
Bioelectric potentials
Biomimetics
Substrates
Fibroblasts
Glutamine
Tissue engineering
Lysine
kalinin
Rats
Skin
peptide A

Keywords

  • collagen
  • cross-linking
  • laminin-332 derived peptides
  • microbial transglutaminase
  • wound healing

Cite this

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title = "In vivo effects of tailored laminin-332 α3 conjugated scaffolds enhances wound healing: a histomorphometric analysis",
abstract = "Surface modification techniques have been used to develop biomimetic scaffolds by incorporating cell adhesion peptides. In our previous work, we have shown the tethering of laminin-332 α3 chain to type I collagen scaffold using microbial transglutaminase (mTGase), promotes cell adhesion, migration, and proliferation. In this study, we evaluated the wound healing properties of tailored laminin-332 α3 chain (peptide A: PPFLMLLKGSTR) tethered to a type I collagen scaffold using mTGase by incorporating transglutaminase substrate peptide sequences containing either glutamine (peptide B: PPFLMLLKGSTREAQQIVM) or lysine (peptide C: PPFLMLLKGSTRKKKKG) in rat full-thickness wound model at two different time points (7 and 21 days). Histological evaluations were assessed for wound closure, epithelialization, angiogenesis, inflammatory, fibroblastic cellular infiltrations, and quantified using stereological methods (p < 0.05). Peptide A and B tethered to collagen scaffold using mTGase stimulated neovascularization, decreased the inflammatory cell infiltration and prominently enhanced the fibroblast proliferation which significantly accelerated the wound healing process. We conclude that surface modification by incorporating motif of laminin-332 α3 chain (peptide A: PPFLMLLK GSTR) domain and transglutaminase substrate to the laminin-332 α3 chain (peptide B: PPFLMLLKGSTREAQQIVM) using mTGase may be a potential candidate for tissue engineering applications and skin regeneration. {\circledC} 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A:2788-2795, 2013.",
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In vivo effects of tailored laminin-332 α3 conjugated scaffolds enhances wound healing : a histomorphometric analysis. / Damodaran, Gopinath; Tiong, William H.C.; Collighan, Russell; Navsaria, Harshad; Griffin, Martin; Pandit, Abhay.

In: Journal of Biomedical Materials Research: Part A, Vol. 101, No. 10, 05.03.2013, p. 2788-2795.

Research output: Contribution to journalArticle

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T2 - Journal of Biomedical Materials Research: Part A

AU - Damodaran, Gopinath

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