In vivo vitamin C supplementation increases phosphoinositol transfer protein expression in peripheral blood mononuclear cells from healthy individuals

Helen R. Griffiths, Rachel S. Willetts, Melissa M. Grant, Nalini Mistry, Joseph Lunec, Ruth J. Bevan

Research output: Contribution to journalArticle

Abstract

Ascorbate can act as both a reducing and oxidising agent in vitro depending on its environment. It can modulate the intracellular redox environment of cells and therefore is predicted to modulate thiol-dependent cell signalling and gene expression pathways. Using proteomic analysis of vitamin C-treated T cells in vitro, we have previously reported changes in expression of five functional protein groups associated with signalling, carbohydrate metabolism, apoptosis, transcription and immune function. The increased expression of the signalling molecule phosphatidylinositol transfer protein (PITP) was also confirmed using Western blotting. Herein, we have compared protein changes elicited by ascorbate in vitro, with the effect of ascorbate on plasma potassium levels, on peripheral blood mononuclear cell (PBMC) apoptosis and PITP expression, in patients supplemented with vitamin C (0-2 g/d) for up to 10 weeks to investigate whether in vitro model systems are predictive of in vivo effects. PITP varied in expression widely between subjects at all time-points analysed but was increased by supplementation with 2 g ascorbate/d after 5 and 10 weeks. No effects on plasma potassium levels were observed in supplemented subjects despite a reduction of K+ channel proteins in ascorbate-treated T cells in vitro. Similarly, no effect of vitamin C supplementation on PBMC apoptosis was observed, whilst ascorbate decreased expression of caspase 3 recruitment domain protein in vitro. These data provide one of the first demonstrations that proteomics may be valuable in developing predictive markers of nutrient effects in vivo and may identify novel pathways for studying mechanisms of action in vivo.
LanguageEnglish
Pages1432-1439
Number of pages8
JournalBritish Journal of Nutrition
Volume101
Issue number10
DOIs
Publication statusPublished - May 2009

Fingerprint

Ascorbic Acid
Blood Cells
Phospholipid Transfer Proteins
Proteins
Apoptosis
Proteomics
Potassium
T-Lymphocytes
Reducing Agents
Carbohydrate Metabolism
Sulfhydryl Compounds
Oxidants
Caspase 3
Oxidation-Reduction
In Vitro Techniques
Western Blotting
Gene Expression
Food

Keywords

  • peripheral blood mononuclear cells
  • proteomics
  • phosphoinositol transfer protein
  • ascorbic acid

Cite this

Griffiths, Helen R. ; Willetts, Rachel S. ; Grant, Melissa M. ; Mistry, Nalini ; Lunec, Joseph ; Bevan, Ruth J. / In vivo vitamin C supplementation increases phosphoinositol transfer protein expression in peripheral blood mononuclear cells from healthy individuals. In: British Journal of Nutrition. 2009 ; Vol. 101, No. 10. pp. 1432-1439.
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In vivo vitamin C supplementation increases phosphoinositol transfer protein expression in peripheral blood mononuclear cells from healthy individuals. / Griffiths, Helen R.; Willetts, Rachel S.; Grant, Melissa M.; Mistry, Nalini; Lunec, Joseph; Bevan, Ruth J.

In: British Journal of Nutrition, Vol. 101, No. 10, 05.2009, p. 1432-1439.

Research output: Contribution to journalArticle

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