Individual variability analysis of fluorescence parameters measured in skin with different levels of nutritive blood flow

Andrey V. Dunaev*, Victor V. Dremin, Evgeny A. Zherebtsov, Ilya E. Rafailov, Karina S. Litvinova, Scott G. Palmer, Neil A. Stewart, Sergei G. Sokolovski, Edik U. Rafailov

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Fluorescence spectroscopy has recently become more common in clinical medicine. However, there are still many unresolved issues related to the methodology and implementation of instruments with this technology. In this study, we aimed to assess individual variability of fluorescence parameters of endogenous markers (NADH, FAD, etc.) measured by fluorescent spectroscopy (FS) in situ and to analyse the factors that lead to a significant scatter of results. Most studied fluorophores have an acceptable scatter of values (mostly up to 30%) for diagnostic purposes. Here we provide evidence that the level of blood volume in tissue impacts FS data with a significant inverse correlation. The distribution function of the fluorescence intensity and the fluorescent contrast coefficient values are a function of the normal distribution for most of the studied fluorophores and the redox ratio. The effects of various physiological (different content of skin melanin) and technical (characteristics of optical filters) factors on the measurement results were additionally studied.The data on the variability of the measurement results in FS should be considered when interpreting the diagnostic parameters, as well as when developing new algorithms for data processing and FS devices.

Original languageEnglish
Pages (from-to)574-583
Number of pages10
JournalMedical Engineering and Physics
Volume37
Issue number6
Early online date24 Apr 2015
DOIs
Publication statusPublished - 2015

Fingerprint

Spectrum Analysis
Skin
Blood
Fluorescence
Spectroscopy
Fluorophores
Melanin
Flavin-Adenine Dinucleotide
Optical filters
Clinical Medicine
Normal Distribution
Fluorescence Spectrometry
Melanins
Fluorescence spectroscopy
Normal distribution
Blood Volume
NAD
Statistical Factor Analysis
Medicine
Oxidation-Reduction

Bibliographical note

Funding: European Community's Seventh Framework Programme (FP7-People-2009-IAPP) under Grant Agreement no. 251531 MEDILASE and by the state task of the Ministry of Education and Science, Russian Federation, for the State University – Education-Science-Production Complex (basic part, no. 310).

Keywords

  • distribution function
  • fluorescence spectroscopy
  • fluorophores
  • individual variability
  • metrological support
  • non-invasive diagnostics
  • physiological scatter

Cite this

Dunaev, Andrey V. ; Dremin, Victor V. ; Zherebtsov, Evgeny A. ; Rafailov, Ilya E. ; Litvinova, Karina S. ; Palmer, Scott G. ; Stewart, Neil A. ; Sokolovski, Sergei G. ; Rafailov, Edik U. / Individual variability analysis of fluorescence parameters measured in skin with different levels of nutritive blood flow. In: Medical Engineering and Physics. 2015 ; Vol. 37, No. 6. pp. 574-583.
@article{af7924f8f8774b37b89c0b2146517e85,
title = "Individual variability analysis of fluorescence parameters measured in skin with different levels of nutritive blood flow",
abstract = "Fluorescence spectroscopy has recently become more common in clinical medicine. However, there are still many unresolved issues related to the methodology and implementation of instruments with this technology. In this study, we aimed to assess individual variability of fluorescence parameters of endogenous markers (NADH, FAD, etc.) measured by fluorescent spectroscopy (FS) in situ and to analyse the factors that lead to a significant scatter of results. Most studied fluorophores have an acceptable scatter of values (mostly up to 30{\%}) for diagnostic purposes. Here we provide evidence that the level of blood volume in tissue impacts FS data with a significant inverse correlation. The distribution function of the fluorescence intensity and the fluorescent contrast coefficient values are a function of the normal distribution for most of the studied fluorophores and the redox ratio. The effects of various physiological (different content of skin melanin) and technical (characteristics of optical filters) factors on the measurement results were additionally studied.The data on the variability of the measurement results in FS should be considered when interpreting the diagnostic parameters, as well as when developing new algorithms for data processing and FS devices.",
keywords = "distribution function, fluorescence spectroscopy, fluorophores, individual variability, metrological support, non-invasive diagnostics, physiological scatter",
author = "Dunaev, {Andrey V.} and Dremin, {Victor V.} and Zherebtsov, {Evgeny A.} and Rafailov, {Ilya E.} and Litvinova, {Karina S.} and Palmer, {Scott G.} and Stewart, {Neil A.} and Sokolovski, {Sergei G.} and Rafailov, {Edik U.}",
note = "Funding: European Community's Seventh Framework Programme (FP7-People-2009-IAPP) under Grant Agreement no. 251531 MEDILASE and by the state task of the Ministry of Education and Science, Russian Federation, for the State University – Education-Science-Production Complex (basic part, no. 310).",
year = "2015",
doi = "10.1016/j.medengphy.2015.03.011",
language = "English",
volume = "37",
pages = "574--583",
journal = "Medical Engineering and Physics",
issn = "1350-4533",
publisher = "Elsevier",
number = "6",

}

Individual variability analysis of fluorescence parameters measured in skin with different levels of nutritive blood flow. / Dunaev, Andrey V.; Dremin, Victor V.; Zherebtsov, Evgeny A.; Rafailov, Ilya E.; Litvinova, Karina S.; Palmer, Scott G.; Stewart, Neil A.; Sokolovski, Sergei G.; Rafailov, Edik U.

In: Medical Engineering and Physics, Vol. 37, No. 6, 2015, p. 574-583.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Individual variability analysis of fluorescence parameters measured in skin with different levels of nutritive blood flow

AU - Dunaev, Andrey V.

AU - Dremin, Victor V.

AU - Zherebtsov, Evgeny A.

AU - Rafailov, Ilya E.

AU - Litvinova, Karina S.

AU - Palmer, Scott G.

AU - Stewart, Neil A.

AU - Sokolovski, Sergei G.

AU - Rafailov, Edik U.

N1 - Funding: European Community's Seventh Framework Programme (FP7-People-2009-IAPP) under Grant Agreement no. 251531 MEDILASE and by the state task of the Ministry of Education and Science, Russian Federation, for the State University – Education-Science-Production Complex (basic part, no. 310).

PY - 2015

Y1 - 2015

N2 - Fluorescence spectroscopy has recently become more common in clinical medicine. However, there are still many unresolved issues related to the methodology and implementation of instruments with this technology. In this study, we aimed to assess individual variability of fluorescence parameters of endogenous markers (NADH, FAD, etc.) measured by fluorescent spectroscopy (FS) in situ and to analyse the factors that lead to a significant scatter of results. Most studied fluorophores have an acceptable scatter of values (mostly up to 30%) for diagnostic purposes. Here we provide evidence that the level of blood volume in tissue impacts FS data with a significant inverse correlation. The distribution function of the fluorescence intensity and the fluorescent contrast coefficient values are a function of the normal distribution for most of the studied fluorophores and the redox ratio. The effects of various physiological (different content of skin melanin) and technical (characteristics of optical filters) factors on the measurement results were additionally studied.The data on the variability of the measurement results in FS should be considered when interpreting the diagnostic parameters, as well as when developing new algorithms for data processing and FS devices.

AB - Fluorescence spectroscopy has recently become more common in clinical medicine. However, there are still many unresolved issues related to the methodology and implementation of instruments with this technology. In this study, we aimed to assess individual variability of fluorescence parameters of endogenous markers (NADH, FAD, etc.) measured by fluorescent spectroscopy (FS) in situ and to analyse the factors that lead to a significant scatter of results. Most studied fluorophores have an acceptable scatter of values (mostly up to 30%) for diagnostic purposes. Here we provide evidence that the level of blood volume in tissue impacts FS data with a significant inverse correlation. The distribution function of the fluorescence intensity and the fluorescent contrast coefficient values are a function of the normal distribution for most of the studied fluorophores and the redox ratio. The effects of various physiological (different content of skin melanin) and technical (characteristics of optical filters) factors on the measurement results were additionally studied.The data on the variability of the measurement results in FS should be considered when interpreting the diagnostic parameters, as well as when developing new algorithms for data processing and FS devices.

KW - distribution function

KW - fluorescence spectroscopy

KW - fluorophores

KW - individual variability

KW - metrological support

KW - non-invasive diagnostics

KW - physiological scatter

UR - http://www.scopus.com/inward/record.url?scp=84928927154&partnerID=8YFLogxK

U2 - 10.1016/j.medengphy.2015.03.011

DO - 10.1016/j.medengphy.2015.03.011

M3 - Article

AN - SCOPUS:84928927154

VL - 37

SP - 574

EP - 583

JO - Medical Engineering and Physics

JF - Medical Engineering and Physics

SN - 1350-4533

IS - 6

ER -