Inositol polyphosphate-mediated iron transport in Pseudomonas aeruginosa

Peter H. Hirst, A.M. Riley, S.J. Mills, Ian D. Spiers, David R. Poyner, Sally Freeman, B.V. Potter, Anthony W. Smith*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

It has previously been shown that myo-inositol hexakisphosphate (myo- InsP6) mediates iron transport into Pseudomonas aeruginosa and overcomes iron-dependent growth inhibition. In this study, the iron transport properties of myo-inositol trisphosphate and tetrakisphosphate regio-isomers were studied. Pseudomonas aeruginosa accumulated iron (III) at similar rates whether complexed with myo-Ins(1,2,3)P3 or myo-InsP6. Iron accumulation from other compounds, notably D/L myo-Ins(1,2,4,5)P4 and another inositol trisphosphate regio-isomer, D-myo-Ins(1,4,5)P3, was dramatically increased. Iron transport profiles from myo-InsP6 into mutants lacking the outer membrane porins oprF, oprD and oprP were similar to the wild-type, indicating that these porins are not involved in the transport process. The rates of reduction of iron (III) to iron (II) complexed to any of the compounds by a Ps. aeruginosa cell lysate were similar, suggesting that a reductive mechanism is not the rate-determining step.

Original languageEnglish
Pages (from-to)537-543
Number of pages7
JournalJournal of Applied Microbiology
Volume86
Issue number3
DOIs
Publication statusPublished - 27 Mar 1999

Keywords

  • biological transport
  • inositol
  • phosphates
  • iron
  • iron radioisotopes
  • NADH
  • NADPH
  • oxidoreductases
  • porins
  • pseudomonas aeruginosa

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