Insulin signalling regulates remating in female drosophila

Stuart Wigby, Cathy Slack, Sebastian Grönke, Pedro Martinez, Federico C.F. Calboli, Tracey Chapman, Linda Partridge

Research output: Contribution to journalArticle

Abstract

Mating rate is a major determinant of female lifespan and fitness, and is predicted to optimize at an intermediate level, beyond which superfluous matings are costly. In female Drosophila melanogaster, nutrition is a key regulator of mating rate but the underlying mechanism is unknown. The evolutionarily conserved insulin/insulin-like growth factor-like signalling (IIS) pathway is responsive to nutrition, and regulates development, metabolism, stress resistance, fecundity and lifespan. Here we show that inhibition of IIS, by ablation of Drosophila insulin-like peptide (DILP)-producing median neurosecretory cells, knockout of dilp2, dilp3 or dilp5 genes, expression of a dominant-negative DILP-receptor (InR) transgene or knockout of Lnk, results in reduced female remating rates. IIS-mediated regulation of female remating can occur independent of virgin receptivity, developmental defects, reduced body size or fecundity, and the receipt of the female receptivity-inhibiting male sex peptide. Our results provide a likely mechanism by which females match remating rates to the perceived nutritional environment. The findings suggest that longevity-mediating genes could often have pleiotropic effects on remating rate. However, overexpression of the IIS-regulated transcription factor dFOXO in the fat body-which extends lifespan- does not affect remating rate. Thus, long life and reduced remating are not obligatorily coupled.

LanguageEnglish
Pages424-431
Number of pages8
JournalProceeding of the Royal Society: Series B
Volume278
Issue number1704
Early online date22 Dec 2010
DOIs
Publication statusPublished - 7 Feb 2011

Fingerprint

Drosophila
insulin
Insulin
Nutrition
peptide
Peptides
Peptide Receptors
Somatomedins
Ablation
Metabolism
Gene expression
Fertility
fecundity
nutrition
Transcription Factors
Genes
Fats
peptides
stress resistance
neurosecretory cells

Bibliographical note

This journal is © 2010 The Royal Society This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords

  • drosophila melanogaster
  • fitness
  • mating and reproduction
  • nutrition
  • sexual selection
  • trade-off

Cite this

Wigby, S., Slack, C., Grönke, S., Martinez, P., Calboli, F. C. F., Chapman, T., & Partridge, L. (2011). Insulin signalling regulates remating in female drosophila. Proceeding of the Royal Society: Series B, 278(1704), 424-431. https://doi.org/10.1098/rspb.2010.1390
Wigby, Stuart ; Slack, Cathy ; Grönke, Sebastian ; Martinez, Pedro ; Calboli, Federico C.F. ; Chapman, Tracey ; Partridge, Linda. / Insulin signalling regulates remating in female drosophila. In: Proceeding of the Royal Society: Series B. 2011 ; Vol. 278, No. 1704. pp. 424-431.
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Wigby, S, Slack, C, Grönke, S, Martinez, P, Calboli, FCF, Chapman, T & Partridge, L 2011, 'Insulin signalling regulates remating in female drosophila' Proceeding of the Royal Society: Series B, vol. 278, no. 1704, pp. 424-431. https://doi.org/10.1098/rspb.2010.1390

Insulin signalling regulates remating in female drosophila. / Wigby, Stuart; Slack, Cathy; Grönke, Sebastian; Martinez, Pedro; Calboli, Federico C.F.; Chapman, Tracey; Partridge, Linda.

In: Proceeding of the Royal Society: Series B, Vol. 278, No. 1704, 07.02.2011, p. 424-431.

Research output: Contribution to journalArticle

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