TY - JOUR
T1 - Integration of a 3D hydrogel matrix within a hollow core photonic crystal fibre for DNA probe immobilization
AU - Rutowska, Monika S.
AU - Garcia Gunning, Fatima C.
AU - Kivlehan, Francine
AU - Moore, Eric
AU - Brennan, Des
AU - Galvin, Paul
AU - Ellis, Andrew D.
N1 - © 2010 IOP Publishing Ltd
PY - 2010/9
Y1 - 2010/9
N2 - In this paper, we demonstrate the integration of a 3D hydrogel matrix within a hollow core photonic crystal fibre (HC-PCF). In addition, we also show the fluorescence of Cy5-labelled DNA molecules immobilized within the hydrogel formed in two different types of HC-PCF. The 3D hydrogel matrix is designed to bind with the amino groups of biomolecules using an appropriate cross-linker, providing higher sensitivity and selectivity than the standard 2D coverage, enabling a greater number of probe molecules to be available per unit area. The HC-PCFs, on the other hand, can be designed to maximize the capture of fluorescence to improve sensitivity and provide longer interaction lengths. This could enable the development of fibre-based point-of-care and remote systems, where the enhanced sensitivity would relax the constraints placed on sources and detectors. In this paper, we will discuss the formation of such polyethylene glycol diacrylate (PEGDA) hydrogels within a HC-PCF, including their optical properties such as light propagation and auto-fluorescence.
AB - In this paper, we demonstrate the integration of a 3D hydrogel matrix within a hollow core photonic crystal fibre (HC-PCF). In addition, we also show the fluorescence of Cy5-labelled DNA molecules immobilized within the hydrogel formed in two different types of HC-PCF. The 3D hydrogel matrix is designed to bind with the amino groups of biomolecules using an appropriate cross-linker, providing higher sensitivity and selectivity than the standard 2D coverage, enabling a greater number of probe molecules to be available per unit area. The HC-PCFs, on the other hand, can be designed to maximize the capture of fluorescence to improve sensitivity and provide longer interaction lengths. This could enable the development of fibre-based point-of-care and remote systems, where the enhanced sensitivity would relax the constraints placed on sources and detectors. In this paper, we will discuss the formation of such polyethylene glycol diacrylate (PEGDA) hydrogels within a HC-PCF, including their optical properties such as light propagation and auto-fluorescence.
UR - http://www.scopus.com/inward/record.url?scp=77958180453&partnerID=8YFLogxK
U2 - 10.1088/0957-0233/21/9/094016
DO - 10.1088/0957-0233/21/9/094016
M3 - Article
SN - 0957-0233
VL - 21
JO - Measurement Science and Technology
JF - Measurement Science and Technology
IS - 9
M1 - 094016
T2 - 20th International Conference on Optical Fibre Sensors
Y2 - 5 October 2009 through 9 October 2009
ER -