Integration of a 3D hydrogel matrix within a hollow core photonic crystal fibre for DNA probe immobilization

Monika S. Rutowska, Fatima C. Garcia Gunning, Francine Kivlehan, Eric Moore, Des Brennan, Paul Galvin, Andrew D. Ellis

Research output: Contribution to journalArticlepeer-review

Abstract

In this paper, we demonstrate the integration of a 3D hydrogel matrix within a hollow core photonic crystal fibre (HC-PCF). In addition, we also show the fluorescence of Cy5-labelled DNA molecules immobilized within the hydrogel formed in two different types of HC-PCF. The 3D hydrogel matrix is designed to bind with the amino groups of biomolecules using an appropriate cross-linker, providing higher sensitivity and selectivity than the standard 2D coverage, enabling a greater number of probe molecules to be available per unit area. The HC-PCFs, on the other hand, can be designed to maximize the capture of fluorescence to improve sensitivity and provide longer interaction lengths. This could enable the development of fibre-based point-of-care and remote systems, where the enhanced sensitivity would relax the constraints placed on sources and detectors. In this paper, we will discuss the formation of such polyethylene glycol diacrylate (PEGDA) hydrogels within a HC-PCF, including their optical properties such as light propagation and auto-fluorescence.
Original languageEnglish
Article number094016
JournalMeasurement Science and Technology
Volume21
Issue number9
Early online date28 Jul 2010
DOIs
Publication statusPublished - Sep 2010
Event20th International Conference on Optical Fibre Sensors - Edinburgh, United Kingdom
Duration: 5 Oct 20099 Oct 2009

Bibliographical note

© 2010 IOP Publishing Ltd

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