Interaction between integrin α9β1 and vascular cell adhesion molecule-1 (VCAM-1) inhibits neutrophil apoptosis

Ewan A. Ross, Mike R. Douglas, Heng Wong See, Emma J. Ross, S. John Curnow, Gerard B. Nash, Ed Rainger, Dagmar Scheel-Toellner, Janet M. Lord, Mike Salmon, Christopher D. Buckley*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

According to the prevailing paradigm, neutrophils are short-lived cells that undergo spontaneous apoptosis within 24 hours of their release from the bone marrow. However, neutrophil survival can be significantly prolonged within inflamed tissue by cytokines, inflammatory mediators, and hypoxia. During screening experiments aimed at identifying the effect of the adhesive microenvironment on neutrophil survival, we found that VCAM-1 (CD106) was able to delay both spontaneous and Fas-induced apoptosis. VCAM-1-mediated survival was as efficient as that induced by the cytokine IFN-β and provided an additive, increased delay in apoptosis when given in combination with IFN-β. VCAM-1 delivered its antiapoptotic effect through binding the integrin α9β1. The α9β 1 signaling pathway shares significant features with the IFN-β survival signaling pathway, requiring PI3 kinase, NF-κB activation, as well as de novo protein synthesis, but the kinetics of NF-κB activation by VCAM-1 were slower and more sustained compared with IFN-β. This study demonstrates a novel functional role for α9β1 in neutrophil biology and suggests that adhesive signaling pathways provide an important extrinsic checkpoint for the resolution of inflammatory responses in tissues.

Original languageEnglish
Pages (from-to)1178-1183
Number of pages6
JournalBlood
Volume107
Issue number3
DOIs
Publication statusPublished - 1 Feb 2006

Bibliographical note

© 2006 by The American Society of Hematology

Fingerprint Dive into the research topics of 'Interaction between integrin α<sub>9</sub>β<sub>1</sub> and vascular cell adhesion molecule-1 (VCAM-1) inhibits neutrophil apoptosis'. Together they form a unique fingerprint.

  • Cite this

    Ross, E. A., Douglas, M. R., See, H. W., Ross, E. J., Curnow, S. J., Nash, G. B., Rainger, E., Scheel-Toellner, D., Lord, J. M., Salmon, M., & Buckley, C. D. (2006). Interaction between integrin α9β1 and vascular cell adhesion molecule-1 (VCAM-1) inhibits neutrophil apoptosis. Blood, 107(3), 1178-1183. https://doi.org/10.1182/blood-2005-07-2692