Label-free detection of hypoxia-induced extracellular vesicle secretion from MCF-7 cells

Tugba Kilic, Ana Teresa De Sousa Valinhas, Ivan Wall, Philippe Renaud, Sandro Carrara

Research output: Contribution to journalArticle

Abstract

Nanoscale extracellular vesicles (EVs) including exosomes (50-150 nm membrane particles) have emerged as promising cancer biomarkers due to the carried genetic information about the parental cells. However the sensitive detection of these vesicles remains a challenge. Here we present a label-free electrochemical sensor to measure the EVs secretion levels of hypoxic and normoxic MCF-7 cells. The sensor design includes two consecutive steps; i) Au electrode surface functionalization for anti-CD81 Antibody and ii) EVs capture. The label-free detection of EVs was done via Differential Pulse Voltammetry (DPV) and Electrochemical Impedance Spectroscopy (EIS). The working linear range for the sensor was 102-109 EVs/ml with an LOD 77 EVs/mL and 379 EVs/ml for EIS and DPV based detection. A blood-abundant protein, RhD was used for the selectivity test. In order to assess the performance of the biosensor, the level of EVs secretion by the human breast cancer MCF-7 cell line was compared with enzyme-linked immunosorbent assays (ELISA) and Nanoparticle Tracking Analysis (NTA). Designed label-free electrochemical sensors utilized for quantification of EVs secretion enhancement due to CoCl2-induced hypoxia and 1.23 fold increase with respect to normoxic conditions was found.

Original languageEnglish
Article number9402
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 20 Jun 2018

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MCF-7 Cells
Dielectric Spectroscopy
Exosomes
Hypoxia
Extracellular Vesicles
Biosensing Techniques
Tumor Biomarkers
Nanoparticles
Blood Proteins
Anti-Idiotypic Antibodies
Electrodes
Enzyme-Linked Immunosorbent Assay
Breast Neoplasms
Cell Line
Membranes

Bibliographical note

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Cite this

Kilic, Tugba ; Valinhas, Ana Teresa De Sousa ; Wall, Ivan ; Renaud, Philippe ; Carrara, Sandro. / Label-free detection of hypoxia-induced extracellular vesicle secretion from MCF-7 cells. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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Label-free detection of hypoxia-induced extracellular vesicle secretion from MCF-7 cells. / Kilic, Tugba; Valinhas, Ana Teresa De Sousa; Wall, Ivan; Renaud, Philippe; Carrara, Sandro.

In: Scientific Reports, Vol. 8, No. 1, 9402 , 20.06.2018.

Research output: Contribution to journalArticle

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