Label-free oligonucleotide biosensor based on dual-peak long period fiber grating

Xianfeng Chen; Chen Liu; Marcos R. Cardoso; Cleber R. Mendonça; David A. Nagel; Anna V. Hine; Lin Zhang

doi:10.1117/12.2207869

Label-free oligonucleotide biosensor based on dual-peak long period fiber grating

Xianfeng Chen
, Chen Liu
, Marcos R. Cardoso
, Cleber R. Mendonça
, David A. Nagel
, Anna V. Hine
, Lin Zhang

Bangor University
Universidade de São Paulo

Research output: Chapter in Book/Published conference output › Conference publication

4 Citations (Scopus)

136 Downloads (Pure)

Abstract

We report the simplification and development of biofunctionalization methodology based on one-step 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)-mediated reaction. The dual-peak long period grating (dLPG) has been demonstrated its inherent ultrahigh sensitivity to refractive index (RI), achieving 50-fold improvement in RI sensitivity over a standard LPG sensor used in low RI range. With the simple and efficient immobilization of unmodified oligonucleotides on sensor surface, dLPG-based biosensor has been used to monitor the hybridization of complementary oligonucleotides showing a detectable oligonucleotide concentration of 4 nM with the advantages of label-free, real-time, and ultrahigh sensitivity.

Original language	English
Title of host publication	Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI
Editors	Israel Gannot
Place of Publication	Bellingham, WA (US)
Publisher	Society of Photo-Optical Instrumentation Engineers (SPIE)
Number of pages	6
ISBN (Print)	978-1-6284-1936-8
DOIs	https://doi.org/10.1117/12.2207869
Publication status	Published - 7 Mar 2016
Event	SPIE Photonics West BiOS 2016 - The Moscone Center, San Francisco, United States Duration: 13 Feb 2016 → 18 Feb 2016

Publication series

Name	SPIE Proceedings
Publisher	SPIE
Volume	9702
ISSN (Print)	0277-786X
ISSN (Electronic)	2410-9045

Conference

Conference	SPIE Photonics West BiOS 2016
Country/Territory	United States
City	San Francisco
Period	13/02/16 → 18/02/16

Bibliographical note

Xianfeng Chen ; Chen Liu ; Marcos R. Cardoso ; Cleber R. Mendonça ; David A. Nagel ; Anna V. Hine and Lin Zhang; "Label-free oligonucleotide biosensor based on dual-peak long period fiber grating", Proc. SPIE 9702, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI, 97020S (March 7, 2016).
Copyright 2016. Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
DOI: http://dx.doi.org/10.1117/12.2207869

Keywords

DNA
EDC-mediated reaction
label-free
long period grating
oligonucleotide
optical fiber biosensor

Access to Document

10.1117/12.2207869

Oligonucleotide biosensor based on dual-peak long period fiber grating
Xianfeng Chen ; Chen Liu ; Marcos R. Cardoso ; Cleber R. Mendonça ; David A. Nagel ; Anna V. Hine and Lin Zhang; "Label-free oligonucleotide biosensor based on dual-peak long period fiber grating", Proc. SPIE 9702, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI, 97020S (March 7, 2016). Copyright 2016. Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. DOI: http://dx.doi.org/10.1117/12.2207869
Final published version, 561 KB

Cite this

Chen, X., Liu, C., Cardoso, M. R., Mendonça, C. R., Nagel, D. A., Hine, A. V., & Zhang, L. (2016). Label-free oligonucleotide biosensor based on dual-peak long period fiber grating. In I. Gannot (Ed.), Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI Article 97020S (SPIE Proceedings; Vol. 9702). Society of Photo-Optical Instrumentation Engineers (SPIE). https://doi.org/10.1117/12.2207869

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abstract = "We report the simplification and development of biofunctionalization methodology based on one-step 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)-mediated reaction. The dual-peak long period grating (dLPG) has been demonstrated its inherent ultrahigh sensitivity to refractive index (RI), achieving 50-fold improvement in RI sensitivity over a standard LPG sensor used in low RI range. With the simple and efficient immobilization of unmodified oligonucleotides on sensor surface, dLPG-based biosensor has been used to monitor the hybridization of complementary oligonucleotides showing a detectable oligonucleotide concentration of 4 nM with the advantages of label-free, real-time, and ultrahigh sensitivity.",

keywords = "DNA, EDC-mediated reaction, label-free, long period grating, oligonucleotide, optical fiber biosensor",

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note = "Xianfeng Chen ; Chen Liu ; Marcos R. Cardoso ; Cleber R. Mendon{\c c}a ; David A. Nagel ; Anna V. Hine and Lin Zhang; {"}Label-free oligonucleotide biosensor based on dual-peak long period fiber grating{"}, Proc. SPIE 9702, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI, 97020S (March 7, 2016). Copyright 2016. Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. DOI: http://dx.doi.org/10.1117/12.2207869 ; SPIE Photonics West BiOS 2016 ; Conference date: 13-02-2016 Through 18-02-2016",

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Chen, X, Liu, C, Cardoso, MR, Mendonça, CR, Nagel, DA , Hine, AV & Zhang, L 2016, Label-free oligonucleotide biosensor based on dual-peak long period fiber grating. in I Gannot (ed.), Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI., 97020S, SPIE Proceedings, vol. 9702, Society of Photo-Optical Instrumentation Engineers (SPIE), Bellingham, WA (US), SPIE Photonics West BiOS 2016, San Francisco, United States, 13/02/16. https://doi.org/10.1117/12.2207869

Label-free oligonucleotide biosensor based on dual-peak long period fiber grating. / Chen, Xianfeng; Liu, Chen; Cardoso, Marcos R. et al.
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI. ed. / Israel Gannot. Bellingham, WA (US): Society of Photo-Optical Instrumentation Engineers (SPIE), 2016. 97020S (SPIE Proceedings; Vol. 9702).

Research output: Chapter in Book/Published conference output › Conference publication

TY - GEN

T1 - Label-free oligonucleotide biosensor based on dual-peak long period fiber grating

AU - Chen, Xianfeng

AU - Liu, Chen

AU - Cardoso, Marcos R.

AU - Mendonça, Cleber R.

AU - Nagel, David A.

AU - Hine, Anna V.

AU - Zhang, Lin

N1 - Xianfeng Chen ; Chen Liu ; Marcos R. Cardoso ; Cleber R. Mendonça ; David A. Nagel ; Anna V. Hine and Lin Zhang; "Label-free oligonucleotide biosensor based on dual-peak long period fiber grating", Proc. SPIE 9702, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI, 97020S (March 7, 2016). Copyright 2016. Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. DOI: http://dx.doi.org/10.1117/12.2207869

PY - 2016/3/7

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N2 - We report the simplification and development of biofunctionalization methodology based on one-step 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)-mediated reaction. The dual-peak long period grating (dLPG) has been demonstrated its inherent ultrahigh sensitivity to refractive index (RI), achieving 50-fold improvement in RI sensitivity over a standard LPG sensor used in low RI range. With the simple and efficient immobilization of unmodified oligonucleotides on sensor surface, dLPG-based biosensor has been used to monitor the hybridization of complementary oligonucleotides showing a detectable oligonucleotide concentration of 4 nM with the advantages of label-free, real-time, and ultrahigh sensitivity.

AB - We report the simplification and development of biofunctionalization methodology based on one-step 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)-mediated reaction. The dual-peak long period grating (dLPG) has been demonstrated its inherent ultrahigh sensitivity to refractive index (RI), achieving 50-fold improvement in RI sensitivity over a standard LPG sensor used in low RI range. With the simple and efficient immobilization of unmodified oligonucleotides on sensor surface, dLPG-based biosensor has been used to monitor the hybridization of complementary oligonucleotides showing a detectable oligonucleotide concentration of 4 nM with the advantages of label-free, real-time, and ultrahigh sensitivity.

KW - DNA

KW - EDC-mediated reaction

KW - label-free

KW - long period grating

KW - oligonucleotide

KW - optical fiber biosensor

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DO - 10.1117/12.2207869

M3 - Conference publication

AN - SCOPUS:84973338417

SN - 978-1-6284-1936-8

T3 - SPIE Proceedings

BT - Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI

A2 - Gannot, Israel

PB - Society of Photo-Optical Instrumentation Engineers (SPIE)

CY - Bellingham, WA (US)

T2 - SPIE Photonics West BiOS 2016

Y2 - 13 February 2016 through 18 February 2016

ER -

Chen X, Liu C, Cardoso MR, Mendonça CR, Nagel DA , Hine AV et al. Label-free oligonucleotide biosensor based on dual-peak long period fiber grating. In Gannot I, editor, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI. Bellingham, WA (US): Society of Photo-Optical Instrumentation Engineers (SPIE). 2016. 97020S. (SPIE Proceedings). doi: 10.1117/12.2207869

Label-free oligonucleotide biosensor based on dual-peak long period fiber grating

Abstract

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Bibliographical note

Keywords

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