Large-scale production of secreted proteins in Pichia pastoris

Nagamani Bora*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Abstract

The production of recombinant therapeutic proteins is an active area of research in drug development. These bio-therapeutic drugs target nearly 150 disease states and promise to bring better treatments to patients. However, if new bio-therapeutics are to be made more accessible and affordable, improvements in production performance and optimization of processes are necessary. A major challenge lies in controlling the effect of process conditions on production of intact functional proteins. To achieve this, improved tools are needed for bio-processing. For example, implementation of process modeling and high-throughput technologies can be used to achieve quality by design, leading to improvements in productivity. Commercially, the most sought after targets are secreted proteins due to the ease of handling in downstream procedures. This chapter outlines different approaches for production and optimization of secreted proteins in the host Pichia pastoris.

Original languageEnglish
Title of host publicationRecombinant protein production in yeast
EditorsRoslyn M. Bill
PublisherHumana Press
Pages217-235
Number of pages19
ISBN (Electronic)978-1-61779-770-5
ISBN (Print)978-1-61779-769-9
DOIs
Publication statusPublished - 25 Feb 2012

Publication series

NameMethods in molecular biology
PublisherHumana Press
Volume866
ISSN (Print)1064-3745
NameSpringer protocols
PublisherSpringer

Keywords

  • bioreactor
  • induction
  • Pichia pastoris
  • promoter
  • secreted protein
  • vector

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  • Research Output

    • 6 Chapter (peer-reviewed)
    • 1 Anthology
    • 1 Foreword/postscript

    Optimising Pichia pastoris induction

    Bawa, Z. & Darby, R. A. J., 25 Feb 2012, Recombinant protein production in yeast: methods and protocols. Bill, R. M. (ed.). Humana Press, p. 181-190 10 p. (Methods in molecular biology; vol. 866)(Springer protocols).

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

  • Optimising yeast as a host for recombinant protein production (review)

    Bonander, N. & Bill, R. M., 25 Feb 2012, Recombinant protein production in yeast: methods and protocols. Bill, R. M. (ed.). Humana Press, p. 1-9 9 p. (Methods in molecular biology; vol. 866)(Springer protocols).

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

  • Preface

    Bill, R. M., 25 Feb 2012, Recombinant protein production in yeast: methods and protocols. Bill, R. M. (ed.). Humana Press, p. vii 1 p. (Methods in molecular biology; vol. 866)(Springer protocols).

    Research output: Chapter in Book/Report/Conference proceedingForeword/postscript

    Cite this

    Bora, N. (2012). Large-scale production of secreted proteins in Pichia pastoris. In R. M. Bill (Ed.), Recombinant protein production in yeast (pp. 217-235). (Methods in molecular biology; Vol. 866), (Springer protocols). Humana Press. https://doi.org/10.1007/978-1-61779-770-5_19