Laser speckle contrast imaging with principal component and entropy analysis: a novel approach for depth-independent blood flow assessment

Yu. Surkov*, P. Timoshina, I. Serebryakova, D. Stavtcev, I. Kozlov, G. Piavchenko, I. Meglinski, A. Konovalov, D. Telyshev, S. Kuznetcov, E. Genina, V. Tuchin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Current study presents an advanced method for improving the visualization of subsurface blood vessels using laser speckle contrast imaging (LSCI), enhanced through principal component analysis (PCA) filtering. By combining LSCI and laser speckle entropy imaging with PCA filtering, the method effectively separates static and dynamic components of the speckle signal, significantly improving the accuracy of blood flow assessments, even in the presence of static scattering layers located above and below the vessel. Experiments conducted on optical phantoms, with the vessel depths ranging from 0.6 to 2 mm, and in vivo studies on a laboratory mouse ear demonstrate substantial improvements in image contrast and resolution. The method’s sensitivity to blood flow velocity within the physiologic range (0.98–19.66 mm/s) is significantly enhanced, while its sensitivity to vessel depth is minimized. These results highlight the method’s ability to assess blood flow velocity independently of vessel depth, overcoming a major limitation of conventional LSCI techniques. The proposed approach holds great potential for non-invasive biomedical imaging, offering improved diagnostic accuracy and contrast in vascular imaging. These findings may be particularly valuable for advancing the use of LSCI in clinical diagnostics and biomedical research, where high precision in blood flow monitoring is essential.
Original languageEnglish
Article number1
Number of pages19
JournalFrontiers of Optoelectronics
Volume18
Early online date3 Jan 2025
DOIs
Publication statusPublished - 3 Jan 2025

Bibliographical note

Copyright © The Author(s) 2025. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/

Keywords

  • Vascular imaging
  • Principal component analysis
  • Entropy
  • Laser speckle imaging
  • Blood flow velocity
  • Speckle contrast

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