Light scattering study of human serum albumin in pre-denaturation: relation to dynamic transition in water at 42°C

Vitaliy Bardik*, Vladimir Gotsulskii, Evgen Pavlov, Nikolai Malomuzh, Dmitry Nerukh, Igor Yanchuk, Serhiy Lavoryk

*Corresponding author for this work

Research output: Contribution to journalSpecial issue

Abstract

Protein functional motions are ultimately connected to water dynamics. The goal of this study is to link the conformational dynamics of albumin to a dynamic transition taking place at ∼ 42°C in water. We report the results of dynamic light scattering measurements of albumin aqueous solution in the temperature interval 20-65°C. The processing of the experimental data produced the temperature dependence of the macromolecular hydrodynamic radius. We demonstrate that the growth of the macromolecular size in this temperature range can be divided into two stages that are connected to the dynamical properties of water.

Original languageEnglish
Pages (from-to)60-64
Number of pages5
JournalJournal of Molecular Liquids
Volume176
Early online date26 Sep 2012
DOIs
Publication statusPublished - Dec 2012
EventEMLG/JMLG 2011 Annual Meeting 11 - 15 September 2011 - , United Kingdom
Duration: 11 Sep 201115 Nov 2011

Fingerprint

Denaturation
biopolymer denaturation
albumins
Serum Albumin
Light scattering
serums
light scattering
Water
Albumins
water
Dynamic light scattering
Temperature
Hydrodynamics
Proteins
hydrodynamics
Processing
aqueous solutions
proteins
intervals
temperature dependence

Bibliographical note

Selected Papers on Molecular Liquids presented at the EMLG/JMLG 2011 Annual Meeting 11 - 15 September 2011

Keywords

  • conformational dynamics
  • dynamic light scattering
  • human serum albumin
  • hydrodynamic radius
  • hydrogen bonds

Cite this

Bardik, Vitaliy ; Gotsulskii, Vladimir ; Pavlov, Evgen ; Malomuzh, Nikolai ; Nerukh, Dmitry ; Yanchuk, Igor ; Lavoryk, Serhiy. / Light scattering study of human serum albumin in pre-denaturation : relation to dynamic transition in water at 42°C. In: Journal of Molecular Liquids. 2012 ; Vol. 176. pp. 60-64.
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author = "Vitaliy Bardik and Vladimir Gotsulskii and Evgen Pavlov and Nikolai Malomuzh and Dmitry Nerukh and Igor Yanchuk and Serhiy Lavoryk",
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Light scattering study of human serum albumin in pre-denaturation : relation to dynamic transition in water at 42°C. / Bardik, Vitaliy; Gotsulskii, Vladimir; Pavlov, Evgen; Malomuzh, Nikolai; Nerukh, Dmitry; Yanchuk, Igor; Lavoryk, Serhiy.

In: Journal of Molecular Liquids, Vol. 176, 12.2012, p. 60-64.

Research output: Contribution to journalSpecial issue

TY - JOUR

T1 - Light scattering study of human serum albumin in pre-denaturation

T2 - relation to dynamic transition in water at 42°C

AU - Bardik, Vitaliy

AU - Gotsulskii, Vladimir

AU - Pavlov, Evgen

AU - Malomuzh, Nikolai

AU - Nerukh, Dmitry

AU - Yanchuk, Igor

AU - Lavoryk, Serhiy

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KW - dynamic light scattering

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