Lipase immobilised on silica monoliths as continuous-flow microreactors for triglyceride transesterification

Mohammed Alotaibi, Jinesh C. Manayil, Gillian M. Greenway, Stephen J. Haswell, Stephen M. Kelly, Adam F. Lee, Karen Wilson, Georgios Kyriakou

Research output: Contribution to journalArticle

Abstract

Lipase immobilised on silica monoliths has been prepared and applied as biocatalytic continuous-flow microreactors for the transesterification of tributyrin as a model bio-oil component. Candida antarctica lipase was trapped within the pores of silica monoliths, and its successful immobilisation was demonstrated by the hydrolysis of 4-nitrophenyl butyrate to 4-nitrophenol. Lipase immobilised on silica monoliths was active for the transesterification of tributyrin at ambient temperature, with reactivity as a function of the methanol : tributyrin ratio, flow rate, temperature, and textural properties. Monoliths with a high surface area and large meso- and macropore channels enhanced the transesterification activity through improved molecule diffusion. The optimum immobilised lipase microreactor exhibited almost quantitative ester production for >100 h at 30 °C without deactivation
Original languageEnglish
Pages (from-to)68-74
JournalReaction Chemistry & Engineering
Volume3
Early online date18 Dec 2017
DOIs
Publication statusPublished - 2018

Bibliographical note

© Royal Society of Chemistry, 2017. Lipase immobilised on silica monoliths as continuous-flow microreactors for triglyceride transesterification
Alotaibi, M., Manayil, J. C., Greenway, G. M., Haswell, S. J., Kelly, S. M., Lee, A. F., Wilson, K. & Kyriakou, G. 18 Dec 2017 In : Reaction Chemistry & Engineering. http://doi.org/10.1039/C7RE00162B

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