Liposomes act as stronger sub-unit vaccine adjuvants when compared to microspheres

Daniel J. Kirby, Ida Rosenkrands, Else M. Agger, Peter Andersen, Allan G. A. Coombes, Yvonne Perrie

Research output: Contribution to journalArticle

Abstract

The ability of liposomes and microspheres to enhance the efficacy of a sub-unit antigen was investigated. Microspheres were optimised by testing a range of surfactants employed in the external aqueous phase of a water-in-oil-in-water (w/o/w) double emulsion solvent evaporation process for the preparation of microspherescomposed of poly(d,l-lactide-co-glycolide) and the immunological adjuvant dimethyl dioctadecyl ammonium bromide (DDA)and then investigated with regard to the physico-chemical and immunological characteristics of the particles produced. The results demonstrate that this parameter can affect the physico-chemical characteristics of these systems and subsequently, has a substantial bearing on the level of immune response achieved, both humoural and cell mediated, when employed for the delivery of the sub-unit tuberculosis vaccine antigen Ag85B-ESAT-6. Moreover, the microsphere preparations investigated failed to initiate immune responses at the levels achieved with an adjuvant DDA-based liposome formulation (DDA-TDB), further substantiating the superior ability of liposomes as vaccine delivery systems.
LanguageEnglish
Pages543-554
Number of pages12
JournalJournal of Drug Targeting
Volume16
Issue number7-8
DOIs
Publication statusPublished - 2008

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Microspheres
Liposomes
Vaccines
Tuberculosis Vaccines
Antigens
Immunologic Adjuvants
Water
Emulsions
Surface-Active Agents
Oils
dimethyldioctadecylammonium

Keywords

  • microspheres
  • liposomes
  • PLGA
  • adjuvants
  • sub-unit vaccines
  • tuberculosis

Cite this

Kirby, D. J., Rosenkrands, I., Agger, E. M., Andersen, P., Coombes, A. G. A., & Perrie, Y. (2008). Liposomes act as stronger sub-unit vaccine adjuvants when compared to microspheres. Journal of Drug Targeting, 16(7-8), 543-554. https://doi.org/10.1080/10611860802228558
Kirby, Daniel J. ; Rosenkrands, Ida ; Agger, Else M. ; Andersen, Peter ; Coombes, Allan G. A. ; Perrie, Yvonne. / Liposomes act as stronger sub-unit vaccine adjuvants when compared to microspheres. In: Journal of Drug Targeting. 2008 ; Vol. 16, No. 7-8. pp. 543-554.
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Kirby, DJ, Rosenkrands, I, Agger, EM, Andersen, P, Coombes, AGA & Perrie, Y 2008, 'Liposomes act as stronger sub-unit vaccine adjuvants when compared to microspheres' Journal of Drug Targeting, vol. 16, no. 7-8, pp. 543-554. https://doi.org/10.1080/10611860802228558

Liposomes act as stronger sub-unit vaccine adjuvants when compared to microspheres. / Kirby, Daniel J.; Rosenkrands, Ida; Agger, Else M.; Andersen, Peter; Coombes, Allan G. A.; Perrie, Yvonne.

In: Journal of Drug Targeting, Vol. 16, No. 7-8, 2008, p. 543-554.

Research output: Contribution to journalArticle

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