Magnetite-doped polydimethylsiloxane (PDMS) for phosphopeptide enrichment

Mairi E. Sandison, K. Tveen Jensen, F. Gesellchen, J.M. Cooper, A.R. Pitt*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Reversible phosphorylation plays a key role in numerous biological processes. Mass spectrometry-based approaches are commonly used to analyze protein phosphorylation, but such analysis is challenging, largely due to the low phosphorylation stoichiometry. Hence, a number of phosphopeptide enrichment strategies have been developed, including metal oxide affinity chromatography (MOAC). Here, we describe a new material for performing MOAC that employs a magnetite-doped polydimethylsiloxane (PDMS), that is suitable for the creation of microwell array and microfluidic systems to enable low volume, high throughput analysis. Incubation time and sample loading were explored and optimized and demonstrate that the embedded magnetite is able to enrich phosphopeptides. This substrate-based approach is rapid, straightforward and suitable for simultaneously performing multiple, low volume enrichments.

Original languageEnglish
Pages (from-to)4974-4981
Number of pages8
JournalAnalyst
Volume139
Issue number19
Early online date21 Jul 2014
DOIs
Publication statusPublished - 7 Oct 2014

Bibliographical note

Funding: RASOR (“Radical Solutions for Researching the proteome”) BBSRC IRC programme (BB/C511572/1) and the Proxomics EPSRC Cross-Disciplinary Research Landscape Award (EP/I017887/1).

Electronic supplementary information: DOI 10.1039/c4an00750f

Fingerprint Dive into the research topics of 'Magnetite-doped polydimethylsiloxane (PDMS) for phosphopeptide enrichment'. Together they form a unique fingerprint.

Cite this