Magnetite-doped polydimethylsiloxane (PDMS) for phosphopeptide enrichment

Mairi E. Sandison, K. Tveen Jensen, F. Gesellchen, J.M. Cooper, A.R. Pitt

Research output: Contribution to journalArticle

Abstract

Reversible phosphorylation plays a key role in numerous biological processes. Mass spectrometry-based approaches are commonly used to analyze protein phosphorylation, but such analysis is challenging, largely due to the low phosphorylation stoichiometry. Hence, a number of phosphopeptide enrichment strategies have been developed, including metal oxide affinity chromatography (MOAC). Here, we describe a new material for performing MOAC that employs a magnetite-doped polydimethylsiloxane (PDMS), that is suitable for the creation of microwell array and microfluidic systems to enable low volume, high throughput analysis. Incubation time and sample loading were explored and optimized and demonstrate that the embedded magnetite is able to enrich phosphopeptides. This substrate-based approach is rapid, straightforward and suitable for simultaneously performing multiple, low volume enrichments.

LanguageEnglish
Pages4974-4981
Number of pages8
JournalAnalyst
Volume139
Issue number19
Early online date21 Jul 2014
DOIs
Publication statusPublished - 7 Oct 2014

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Ferrosoferric Oxide
Phosphopeptides
Phosphorylation
Magnetite
Polydimethylsiloxane
chromatography
Affinity chromatography
magnetite
Affinity Chromatography
Oxides
stoichiometry
Metals
biological processes
Biological Phenomena
Microfluidics
mass spectrometry
incubation
substrate
Stoichiometry
protein

Bibliographical note

Funding: RASOR (“Radical Solutions for Researching the proteome”) BBSRC IRC programme (BB/C511572/1) and the Proxomics EPSRC Cross-Disciplinary Research Landscape Award (EP/I017887/1).

Electronic supplementary information: DOI 10.1039/c4an00750f

Cite this

Sandison, Mairi E. ; Tveen Jensen, K. ; Gesellchen, F. ; Cooper, J.M. ; Pitt, A.R. / Magnetite-doped polydimethylsiloxane (PDMS) for phosphopeptide enrichment. In: Analyst. 2014 ; Vol. 139, No. 19. pp. 4974-4981.
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Magnetite-doped polydimethylsiloxane (PDMS) for phosphopeptide enrichment. / Sandison, Mairi E.; Tveen Jensen, K.; Gesellchen, F.; Cooper, J.M.; Pitt, A.R.

In: Analyst, Vol. 139, No. 19, 07.10.2014, p. 4974-4981.

Research output: Contribution to journalArticle

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