Manufacturing methods for liposome adjuvants

Yvonne Perrie, Elisabeth Kastner, Swapnil Khadke, Carla B. Roces, Peter Stone

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A wide range of studies have shown that liposomes can act as suitable adjuvants for a range of vaccine antigens. Properties such as their amphiphilic character and biphasic nature allow them to incorporate antigens within the lipid bilayer, on the surface, or encapsulated within the inner core. However, appropriate methods for the manufacture of liposomes are limited and this has resulted in issues with cost, supply, and wider scale application of these systems. Within this chapter we explore manufacturing processes that can be used for the production of liposomal adjuvants, and we outline new manufacturing methods can that offer fast, scalable, and cost-effective production of liposomal adjuvants.

LanguageEnglish
Title of host publicationVaccine adjuvants
Subtitle of host publicationmethods and protocols
EditorsChristopher B. Fox
Place of PublicationNew York (US)
PublisherSpringer
Pages127-144
Number of pages18
ISBN (Electronic)978-1-4939-6445-1
ISBN (Print)978-1-4939-6443-7
DOIs
Publication statusPublished - 8 Oct 2016

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Volume1494
ISSN (Print)1064-3745

Fingerprint

Liposomes
Antigens
Costs and Cost Analysis
Lipid Bilayers
Vaccines

Keywords

  • adjuvants
  • delivery system
  • liposomes
  • manufacturing
  • preparation
  • subunit vaccine

Cite this

Perrie, Y., Kastner, E., Khadke, S., Roces, C. B., & Stone, P. (2016). Manufacturing methods for liposome adjuvants. In C. B. Fox (Ed.), Vaccine adjuvants: methods and protocols (pp. 127-144). (Methods in Molecular Biology; Vol. 1494). New York (US): Springer. https://doi.org/10.1007/978-1-4939-6445-1_9
Perrie, Yvonne ; Kastner, Elisabeth ; Khadke, Swapnil ; Roces, Carla B. ; Stone, Peter. / Manufacturing methods for liposome adjuvants. Vaccine adjuvants: methods and protocols. editor / Christopher B. Fox. New York (US) : Springer, 2016. pp. 127-144 (Methods in Molecular Biology).
@inbook{d0946f85bcf14a51932c681fadea6bbf,
title = "Manufacturing methods for liposome adjuvants",
abstract = "A wide range of studies have shown that liposomes can act as suitable adjuvants for a range of vaccine antigens. Properties such as their amphiphilic character and biphasic nature allow them to incorporate antigens within the lipid bilayer, on the surface, or encapsulated within the inner core. However, appropriate methods for the manufacture of liposomes are limited and this has resulted in issues with cost, supply, and wider scale application of these systems. Within this chapter we explore manufacturing processes that can be used for the production of liposomal adjuvants, and we outline new manufacturing methods can that offer fast, scalable, and cost-effective production of liposomal adjuvants.",
keywords = "adjuvants, delivery system, liposomes, manufacturing, preparation, subunit vaccine",
author = "Yvonne Perrie and Elisabeth Kastner and Swapnil Khadke and Roces, {Carla B.} and Peter Stone",
year = "2016",
month = "10",
day = "8",
doi = "10.1007/978-1-4939-6445-1_9",
language = "English",
isbn = "978-1-4939-6443-7",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "127--144",
editor = "Fox, {Christopher B.}",
booktitle = "Vaccine adjuvants",
address = "Germany",

}

Perrie, Y, Kastner, E, Khadke, S, Roces, CB & Stone, P 2016, Manufacturing methods for liposome adjuvants. in CB Fox (ed.), Vaccine adjuvants: methods and protocols. Methods in Molecular Biology, vol. 1494, Springer, New York (US), pp. 127-144. https://doi.org/10.1007/978-1-4939-6445-1_9

Manufacturing methods for liposome adjuvants. / Perrie, Yvonne; Kastner, Elisabeth; Khadke, Swapnil; Roces, Carla B.; Stone, Peter.

Vaccine adjuvants: methods and protocols. ed. / Christopher B. Fox. New York (US) : Springer, 2016. p. 127-144 (Methods in Molecular Biology; Vol. 1494).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Manufacturing methods for liposome adjuvants

AU - Perrie, Yvonne

AU - Kastner, Elisabeth

AU - Khadke, Swapnil

AU - Roces, Carla B.

AU - Stone, Peter

PY - 2016/10/8

Y1 - 2016/10/8

N2 - A wide range of studies have shown that liposomes can act as suitable adjuvants for a range of vaccine antigens. Properties such as their amphiphilic character and biphasic nature allow them to incorporate antigens within the lipid bilayer, on the surface, or encapsulated within the inner core. However, appropriate methods for the manufacture of liposomes are limited and this has resulted in issues with cost, supply, and wider scale application of these systems. Within this chapter we explore manufacturing processes that can be used for the production of liposomal adjuvants, and we outline new manufacturing methods can that offer fast, scalable, and cost-effective production of liposomal adjuvants.

AB - A wide range of studies have shown that liposomes can act as suitable adjuvants for a range of vaccine antigens. Properties such as their amphiphilic character and biphasic nature allow them to incorporate antigens within the lipid bilayer, on the surface, or encapsulated within the inner core. However, appropriate methods for the manufacture of liposomes are limited and this has resulted in issues with cost, supply, and wider scale application of these systems. Within this chapter we explore manufacturing processes that can be used for the production of liposomal adjuvants, and we outline new manufacturing methods can that offer fast, scalable, and cost-effective production of liposomal adjuvants.

KW - adjuvants

KW - delivery system

KW - liposomes

KW - manufacturing

KW - preparation

KW - subunit vaccine

UR - http://www.scopus.com/inward/record.url?scp=84991204023&partnerID=8YFLogxK

UR - https://link.springer.com/protocol/10.1007%2F978-1-4939-6445-1_9

U2 - 10.1007/978-1-4939-6445-1_9

DO - 10.1007/978-1-4939-6445-1_9

M3 - Chapter

SN - 978-1-4939-6443-7

T3 - Methods in Molecular Biology

SP - 127

EP - 144

BT - Vaccine adjuvants

A2 - Fox, Christopher B.

PB - Springer

CY - New York (US)

ER -

Perrie Y, Kastner E, Khadke S, Roces CB, Stone P. Manufacturing methods for liposome adjuvants. In Fox CB, editor, Vaccine adjuvants: methods and protocols. New York (US): Springer. 2016. p. 127-144. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6445-1_9