Matrix-free mass spectrometric imaging using laser desorption ionisation Fourier transform ion cyclotron resonance mass spectrometry

Richard J.A. Goodwin, Andrew R. Pitt, David Harrison, Stefan K. Weidt, Pat R.R. Langridge-Smith, Michael P. Barrett, C. Logan Mackay

Research output: Contribution to journalArticle

Abstract

Mass spectrometry imaging (MSI) is a powerful tool in metabolomics and proteomics for the spatial localization and identification of pharmaceuticals, metabolites, lipids, peptides and proteins in biological tissues. However, sample preparation remains a crucial variable in obtaining the most accurate distributions. Common washing steps used to remove salts, and solvent-based matrix application, allow analyte spreading to occur. Solvent-free matrix applications can reduce this risk, but increase the possibility of ionisation bias due to matrix adhesion to tissue sections. We report here the use of matrix-free MSI using laser desorption ionisation performed on a 12 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. We used unprocessed tissue with no post-processing following thaw-mounting on matrix-assisted laser desorption ionisation (MALDI) indium-tin oxide (ITO) target plates. The identification and distribution of a range of phospholipids in mouse brain and kidney sections are presented and compared with previously published MALDI time-of-flight (TOF) MSI distributions.
LanguageEnglish
Pages969-72
Number of pages4
JournalRapid communications in mass spectrometry
Volume25
Issue number7
Early online date14 Mar 2011
DOIs
Publication statusPublished - 15 Apr 2011

Fingerprint

Cyclotron resonance
Ionization
Mass spectrometry
Desorption
Fourier transforms
Ions
Imaging techniques
Lasers
Tissue
Mass spectrometers
Metabolites
Mountings
Washing
Brain
Phospholipids
Adhesion
Salts
Lipids
Peptides
Processing

Keywords

  • animals
  • brain chemistry
  • Fourier analysis
  • histocytochemistry
  • kidney
  • metabolomics
  • mice
  • phospholipids
  • spectrometry
  • tin compounds
  • mass
  • matrix-assisted laser desorption-ionization

Cite this

Goodwin, Richard J.A. ; Pitt, Andrew R. ; Harrison, David ; Weidt, Stefan K. ; Langridge-Smith, Pat R.R. ; Barrett, Michael P. ; Mackay, C. Logan. / Matrix-free mass spectrometric imaging using laser desorption ionisation Fourier transform ion cyclotron resonance mass spectrometry. In: Rapid communications in mass spectrometry. 2011 ; Vol. 25, No. 7. pp. 969-72.
@article{8eb3cb70bc5242a2a4423d1150fc3bbc,
title = "Matrix-free mass spectrometric imaging using laser desorption ionisation Fourier transform ion cyclotron resonance mass spectrometry",
abstract = "Mass spectrometry imaging (MSI) is a powerful tool in metabolomics and proteomics for the spatial localization and identification of pharmaceuticals, metabolites, lipids, peptides and proteins in biological tissues. However, sample preparation remains a crucial variable in obtaining the most accurate distributions. Common washing steps used to remove salts, and solvent-based matrix application, allow analyte spreading to occur. Solvent-free matrix applications can reduce this risk, but increase the possibility of ionisation bias due to matrix adhesion to tissue sections. We report here the use of matrix-free MSI using laser desorption ionisation performed on a 12 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. We used unprocessed tissue with no post-processing following thaw-mounting on matrix-assisted laser desorption ionisation (MALDI) indium-tin oxide (ITO) target plates. The identification and distribution of a range of phospholipids in mouse brain and kidney sections are presented and compared with previously published MALDI time-of-flight (TOF) MSI distributions.",
keywords = "animals, brain chemistry, Fourier analysis, histocytochemistry, kidney, metabolomics, mice, phospholipids, spectrometry, tin compounds, mass, matrix-assisted laser desorption-ionization",
author = "Goodwin, {Richard J.A.} and Pitt, {Andrew R.} and David Harrison and Weidt, {Stefan K.} and Langridge-Smith, {Pat R.R.} and Barrett, {Michael P.} and Mackay, {C. Logan}",
note = "Copyright {\circledC} 2011 John Wiley & Sons, Ltd.",
year = "2011",
month = "4",
day = "15",
doi = "10.1002/rcm.4939",
language = "English",
volume = "25",
pages = "969--72",
journal = "Rapid communications in mass spectrometry",
issn = "0951-4198",
publisher = "John Wiley and Sons Ltd",
number = "7",

}

Matrix-free mass spectrometric imaging using laser desorption ionisation Fourier transform ion cyclotron resonance mass spectrometry. / Goodwin, Richard J.A.; Pitt, Andrew R.; Harrison, David; Weidt, Stefan K.; Langridge-Smith, Pat R.R.; Barrett, Michael P.; Mackay, C. Logan.

In: Rapid communications in mass spectrometry, Vol. 25, No. 7, 15.04.2011, p. 969-72.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Matrix-free mass spectrometric imaging using laser desorption ionisation Fourier transform ion cyclotron resonance mass spectrometry

AU - Goodwin, Richard J.A.

AU - Pitt, Andrew R.

AU - Harrison, David

AU - Weidt, Stefan K.

AU - Langridge-Smith, Pat R.R.

AU - Barrett, Michael P.

AU - Mackay, C. Logan

N1 - Copyright © 2011 John Wiley & Sons, Ltd.

PY - 2011/4/15

Y1 - 2011/4/15

N2 - Mass spectrometry imaging (MSI) is a powerful tool in metabolomics and proteomics for the spatial localization and identification of pharmaceuticals, metabolites, lipids, peptides and proteins in biological tissues. However, sample preparation remains a crucial variable in obtaining the most accurate distributions. Common washing steps used to remove salts, and solvent-based matrix application, allow analyte spreading to occur. Solvent-free matrix applications can reduce this risk, but increase the possibility of ionisation bias due to matrix adhesion to tissue sections. We report here the use of matrix-free MSI using laser desorption ionisation performed on a 12 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. We used unprocessed tissue with no post-processing following thaw-mounting on matrix-assisted laser desorption ionisation (MALDI) indium-tin oxide (ITO) target plates. The identification and distribution of a range of phospholipids in mouse brain and kidney sections are presented and compared with previously published MALDI time-of-flight (TOF) MSI distributions.

AB - Mass spectrometry imaging (MSI) is a powerful tool in metabolomics and proteomics for the spatial localization and identification of pharmaceuticals, metabolites, lipids, peptides and proteins in biological tissues. However, sample preparation remains a crucial variable in obtaining the most accurate distributions. Common washing steps used to remove salts, and solvent-based matrix application, allow analyte spreading to occur. Solvent-free matrix applications can reduce this risk, but increase the possibility of ionisation bias due to matrix adhesion to tissue sections. We report here the use of matrix-free MSI using laser desorption ionisation performed on a 12 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. We used unprocessed tissue with no post-processing following thaw-mounting on matrix-assisted laser desorption ionisation (MALDI) indium-tin oxide (ITO) target plates. The identification and distribution of a range of phospholipids in mouse brain and kidney sections are presented and compared with previously published MALDI time-of-flight (TOF) MSI distributions.

KW - animals

KW - brain chemistry

KW - Fourier analysis

KW - histocytochemistry

KW - kidney

KW - metabolomics

KW - mice

KW - phospholipids

KW - spectrometry

KW - tin compounds

KW - mass

KW - matrix-assisted laser desorption-ionization

UR - http://www.scopus.com/inward/record.url?scp=79952804250&partnerID=8YFLogxK

UR - http://onlinelibrary.wiley.com/doi/10.1002/rcm.4939/abstract

U2 - 10.1002/rcm.4939

DO - 10.1002/rcm.4939

M3 - Article

VL - 25

SP - 969

EP - 972

JO - Rapid communications in mass spectrometry

T2 - Rapid communications in mass spectrometry

JF - Rapid communications in mass spectrometry

SN - 0951-4198

IS - 7

ER -