Matrix-free mass spectrometric imaging using laser desorption ionisation Fourier transform ion cyclotron resonance mass spectrometry

Richard J.A. Goodwin, Andrew R. Pitt, David Harrison, Stefan K. Weidt, Pat R.R. Langridge-Smith, Michael P. Barrett, C. Logan Mackay

Research output: Contribution to journalArticle

Abstract

Mass spectrometry imaging (MSI) is a powerful tool in metabolomics and proteomics for the spatial localization and identification of pharmaceuticals, metabolites, lipids, peptides and proteins in biological tissues. However, sample preparation remains a crucial variable in obtaining the most accurate distributions. Common washing steps used to remove salts, and solvent-based matrix application, allow analyte spreading to occur. Solvent-free matrix applications can reduce this risk, but increase the possibility of ionisation bias due to matrix adhesion to tissue sections. We report here the use of matrix-free MSI using laser desorption ionisation performed on a 12 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. We used unprocessed tissue with no post-processing following thaw-mounting on matrix-assisted laser desorption ionisation (MALDI) indium-tin oxide (ITO) target plates. The identification and distribution of a range of phospholipids in mouse brain and kidney sections are presented and compared with previously published MALDI time-of-flight (TOF) MSI distributions.
Original languageEnglish
Pages (from-to)969-72
Number of pages4
JournalRapid communications in mass spectrometry
Volume25
Issue number7
Early online date14 Mar 2011
DOIs
Publication statusPublished - 15 Apr 2011

Keywords

  • animals
  • brain chemistry
  • Fourier analysis
  • histocytochemistry
  • kidney
  • metabolomics
  • mice
  • phospholipids
  • spectrometry
  • tin compounds
  • mass
  • matrix-assisted laser desorption-ionization

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