Medida del colesterol de lipoproteínas de baja densidad utilizando tres metodologías: Medición de colesterol de lipoproteínas de baja densidad

Translated title of the contribution: Measurement of cholesterol low-density lipoprotein using three methodologies

Marvin Querales, María Elena Cruces, César Sánchez, Marisel Querales, Susan Rojas, Lissette Sanchez Aranguren

Research output: Contribution to journalArticlepeer-review

Abstract

Considering that there is still no standard methodology for routine determination of low density lipoprotein (LDL-c) it was decided to evaluate their analytical determination using three techniques: homogeneous enzymatic
determination, polyvinyl sulphate precipitation and Friedewald formula. Ninety-eight serum samples were processed; triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL-c) and LDL-c were determined. Mean total cholesterol was 194.46 ± 43.54 mg/dL, HDL-C was 51.12 ± 12.36 mg/dL and TG was 132.88 ± 76.93 mg/dL. Although regression analysis showed a good
correlation between LDL-c, the results showed a statistically significative difference in them when TG levels exceeded 200 mg/dL. It was mainly observed in the precipitation method and the Friedewald formula, the latter values being significantly lower (LDL-C by precipitation: 141.3 ± 26.2 mg/dL, LDL-C by the
Friedewald formula: 110, 1 ± 35.4 mg/dL). Moreover, this difference affected the proportion of individuals classified according to their coronary risk. It is necessary to compare the techniques applied in this study with beta quantification to assess which has a higher level of accuracy.
Translated title of the contributionMeasurement of cholesterol low-density lipoprotein using three methodologies
Original languageSpanish
Pages (from-to)31-37
JournalActa Bioquimica Clinica Latinoamericana
Volume46
Issue number1
Publication statusPublished - 2012

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