Abstract
Interleukin-11 (IL-11) is a polyfunctional cytokine whose biological actions require a specific IL-11 receptor (IL-11R) and the transmembrane transducer gp130. Here we report the production of a soluble form of the murine IL-11R and demonstrate that it interacts with IL-11 ligand with high affinity. The affinity of IL-11 alone for gp130 is below the level of detection, but a complex of IL-11 and soluble IL-11R interacts with gp130 with high affinity. The addition of soluble IL-11R potentiates the effects of exogenous IL-11 in cells that are normally responsive to IL-11. A biological response to IL-11 can be reconstituted in BAF cells transfected with gp130 by addition of IL-11 and soluble IL-11R. These findings show that the cytoplasmic domain of the IL-11R is not required for the biological effects of IL-11 and that a complex of IL-11 and IL-11R mediates signalling by association with gp130.
| Original language | English |
|---|---|
| Pages (from-to) | 489-95 |
| Number of pages | 7 |
| Journal | Biochemical Journal |
| Volume | 318 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1 Sept 1996 |
Keywords
- Animals
- Antigens, CD
- B-Lymphocytes
- Cell Division
- Cell Line
- Cytokine Receptor gp130
- Electrophoresis, Polyacrylamide Gel
- Genetic Vectors
- Humans
- Interleukin-11
- Interleukin-11 Receptor alpha Subunit
- Kinetics
- Membrane Glycoproteins
- Mice
- Polymerase Chain Reaction
- Receptors, Interleukin
- Receptors, Interleukin-11
- Recombinant Proteins
- Signal Transduction
- Transfection