Mediation of interleukin-11-dependent biological responses by a soluble form of the interleukin-11 receptor

J Karow, K R Hudson, M A Hall, Ann Vernallis, J A Taylor, A Gossler, J K Heath

Research output: Contribution to journalArticle

Abstract

Interleukin-11 (IL-11) is a polyfunctional cytokine whose biological actions require a specific IL-11 receptor (IL-11R) and the transmembrane transducer gp130. Here we report the production of a soluble form of the murine IL-11R and demonstrate that it interacts with IL-11 ligand with high affinity. The affinity of IL-11 alone for gp130 is below the level of detection, but a complex of IL-11 and soluble IL-11R interacts with gp130 with high affinity. The addition of soluble IL-11R potentiates the effects of exogenous IL-11 in cells that are normally responsive to IL-11. A biological response to IL-11 can be reconstituted in BAF cells transfected with gp130 by addition of IL-11 and soluble IL-11R. These findings show that the cytoplasmic domain of the IL-11R is not required for the biological effects of IL-11 and that a complex of IL-11 and IL-11R mediates signalling by association with gp130.
Original languageEnglish
Pages (from-to)489-95
Number of pages7
JournalBiochemical Journal
Volume318
Issue number2
DOIs
Publication statusPublished - 1 Sep 1996

Keywords

  • Animals
  • Antigens, CD
  • B-Lymphocytes
  • Cell Division
  • Cell Line
  • Cytokine Receptor gp130
  • Electrophoresis, Polyacrylamide Gel
  • Genetic Vectors
  • Humans
  • Interleukin-11
  • Interleukin-11 Receptor alpha Subunit
  • Kinetics
  • Membrane Glycoproteins
  • Mice
  • Polymerase Chain Reaction
  • Receptors, Interleukin
  • Receptors, Interleukin-11
  • Recombinant Proteins
  • Signal Transduction
  • Transfection

Fingerprint Dive into the research topics of 'Mediation of interleukin-11-dependent biological responses by a soluble form of the interleukin-11 receptor'. Together they form a unique fingerprint.

  • Cite this