TY - JOUR
T1 - Meningiomas expressing and responding to cholecystokinin (CCK)
AU - Oikonomou, Eftychia
AU - Machado, Ana L.
AU - Buchfelder, Michael
AU - Adams, Eric F.
PY - 2005/7
Y1 - 2005/7
N2 - The effect of cholecystokinin (CCK) on cultured human meningioma derived cells was investigated. Exposure of meningioma cells for 6-12 days to CCK-8s (2-200 nM) resulted in a dose dependent stimulation of cell growth to a maximum of 1.1-fold over basal controls. A time course study showed stimulation of cell growth at day 3 followed by increase throughout day 6. The stimulatory effect of CCK on meningioma cell growth was completely abolished by a CCK-B specific receptor antagonist, L-365,260. Reverse-transcription of meningioma-derived RNA into cDNA followed by amplification by the polymerase chain reaction using specific primers for CCK peptide and its CCK-A and/B receptor revealed 100% presence of CCK peptide and CCK-B receptors mRNA whereas CCK-A receptor was expressed in 66% of the meningiomas. These results provide evidence that human meningioma cells possess CCK peptide and its receptors the activation of which leads to increase of cell growth possibly via an autocrine/paracrine mechanism. © Springer 2005.
AB - The effect of cholecystokinin (CCK) on cultured human meningioma derived cells was investigated. Exposure of meningioma cells for 6-12 days to CCK-8s (2-200 nM) resulted in a dose dependent stimulation of cell growth to a maximum of 1.1-fold over basal controls. A time course study showed stimulation of cell growth at day 3 followed by increase throughout day 6. The stimulatory effect of CCK on meningioma cell growth was completely abolished by a CCK-B specific receptor antagonist, L-365,260. Reverse-transcription of meningioma-derived RNA into cDNA followed by amplification by the polymerase chain reaction using specific primers for CCK peptide and its CCK-A and/B receptor revealed 100% presence of CCK peptide and CCK-B receptors mRNA whereas CCK-A receptor was expressed in 66% of the meningiomas. These results provide evidence that human meningioma cells possess CCK peptide and its receptors the activation of which leads to increase of cell growth possibly via an autocrine/paracrine mechanism. © Springer 2005.
KW - CCK-A and/B antagonist CCK Receptors
KW - cholecystokinin
KW - epidermal growth factor
KW - meningioma
UR - http://www.scopus.com/inward/record.url?scp=22144459406&partnerID=8YFLogxK
UR - http://link.springer.com/article/10.1007%2Fs11060-004-5177-2
U2 - 10.1007/s11060-004-5177-2
DO - 10.1007/s11060-004-5177-2
M3 - Article
C2 - 15980969
SN - 0167-594X
VL - 73
SP - 199
EP - 204
JO - Journal of Neuro-Oncology
JF - Journal of Neuro-Oncology
IS - 3
ER -