Metabolism of vitamin E acetate by reconstituted human gingival and buccal epithelium

A. E. Scott, J. Alcock, M. J. Carlile*, H. R. Griffiths

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Aim: To demonstrate metabolism of vitamin E acetate to vitamin E by human gingival and buccal reconstituted epithelial cell cultures. Method: Human gingival and buccal reconstituted epithelial cell cultures (SkinEthic, France) were exposed to vitamin E acetate and maintained for up to seven days at 37°C (5% CO2) in a humidified incubator. Following organic extraction of the cultures, reverse phase HPLC was used to analyse the quantities of vitamin E and vitamin E acetate in the cultures. Results: The ratio of vitamin E to vitamin E acetate increased up to 30 fold more than the solution control in gingival cultures after 7 days. Conclusion: This paper demonstrates that human gingival and buccal epithelial cells can metabolise vitamin E acetate to vitamin E, thus delivering the more active vitamin E species under the control of the activity of endogenous host enzymes.

Original languageEnglish
Pages (from-to)135-139
Number of pages5
JournalInternational Dental Journal
Volume57
Issue number2 SUPPL. 1
Publication statusPublished - 30 Apr 2007

Fingerprint

Cheek
Vitamin E
Acetates
Epithelium
Tocopherols
Epithelial Cells
Cell Culture Techniques
Incubators
France
High Pressure Liquid Chromatography
Enzymes

Keywords

  • Buccal
  • Gingival tissue
  • Reconstituted epithelium
  • Tocopherol
  • Tocopherol acetate
  • Vitamin E
  • Vitamin E acetate

Cite this

Scott, A. E., Alcock, J., Carlile, M. J., & Griffiths, H. R. (2007). Metabolism of vitamin E acetate by reconstituted human gingival and buccal epithelium. International Dental Journal, 57(2 SUPPL. 1), 135-139.
Scott, A. E. ; Alcock, J. ; Carlile, M. J. ; Griffiths, H. R. / Metabolism of vitamin E acetate by reconstituted human gingival and buccal epithelium. In: International Dental Journal. 2007 ; Vol. 57, No. 2 SUPPL. 1. pp. 135-139.
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Scott, AE, Alcock, J, Carlile, MJ & Griffiths, HR 2007, 'Metabolism of vitamin E acetate by reconstituted human gingival and buccal epithelium', International Dental Journal, vol. 57, no. 2 SUPPL. 1, pp. 135-139.

Metabolism of vitamin E acetate by reconstituted human gingival and buccal epithelium. / Scott, A. E.; Alcock, J.; Carlile, M. J.; Griffiths, H. R.

In: International Dental Journal, Vol. 57, No. 2 SUPPL. 1, 30.04.2007, p. 135-139.

Research output: Contribution to journalArticle

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AU - Scott, A. E.

AU - Alcock, J.

AU - Carlile, M. J.

AU - Griffiths, H. R.

PY - 2007/4/30

Y1 - 2007/4/30

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AB - Aim: To demonstrate metabolism of vitamin E acetate to vitamin E by human gingival and buccal reconstituted epithelial cell cultures. Method: Human gingival and buccal reconstituted epithelial cell cultures (SkinEthic, France) were exposed to vitamin E acetate and maintained for up to seven days at 37°C (5% CO2) in a humidified incubator. Following organic extraction of the cultures, reverse phase HPLC was used to analyse the quantities of vitamin E and vitamin E acetate in the cultures. Results: The ratio of vitamin E to vitamin E acetate increased up to 30 fold more than the solution control in gingival cultures after 7 days. Conclusion: This paper demonstrates that human gingival and buccal epithelial cells can metabolise vitamin E acetate to vitamin E, thus delivering the more active vitamin E species under the control of the activity of endogenous host enzymes.

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Scott AE, Alcock J, Carlile MJ, Griffiths HR. Metabolism of vitamin E acetate by reconstituted human gingival and buccal epithelium. International Dental Journal. 2007 Apr 30;57(2 SUPPL. 1):135-139.