Metabolism of vitamin E acetate by reconstituted human gingival and buccal epithelium

A. E. Scott, J. Alcock, M. J. Carlile*, H. R. Griffiths

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Aim: To demonstrate metabolism of vitamin E acetate to vitamin E by human gingival and buccal reconstituted epithelial cell cultures. Method: Human gingival and buccal reconstituted epithelial cell cultures (SkinEthic, France) were exposed to vitamin E acetate and maintained for up to seven days at 37°C (5% CO2) in a humidified incubator. Following organic extraction of the cultures, reverse phase HPLC was used to analyse the quantities of vitamin E and vitamin E acetate in the cultures. Results: The ratio of vitamin E to vitamin E acetate increased up to 30 fold more than the solution control in gingival cultures after 7 days. Conclusion: This paper demonstrates that human gingival and buccal epithelial cells can metabolise vitamin E acetate to vitamin E, thus delivering the more active vitamin E species under the control of the activity of endogenous host enzymes.

Original languageEnglish
Pages (from-to)135-139
Number of pages5
JournalInternational Dental Journal
Volume57
Issue number2 SUPPL. 1
Publication statusPublished - 30 Apr 2007

Keywords

  • Buccal
  • Gingival tissue
  • Reconstituted epithelium
  • Tocopherol
  • Tocopherol acetate
  • Vitamin E
  • Vitamin E acetate

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    Scott, A. E., Alcock, J., Carlile, M. J., & Griffiths, H. R. (2007). Metabolism of vitamin E acetate by reconstituted human gingival and buccal epithelium. International Dental Journal, 57(2 SUPPL. 1), 135-139.