TY - JOUR
T1 - Methods available for the sub-typing of Escherichia coli O157
AU - Hopkins, Katie L.
AU - Hilton, Anthony C.
PY - 2000
Y1 - 2000
N2 - The epidemiological investigation of Escherichia coli O157 is complicated by the lack of heterogeneity between strains responsible for the majority of cases of infection. As a consequence it is difficult to reliably cluster together an outbreak strain and differentiate it from other sporadically occurring isolates. The methods available for the subtyping of E. coli O157 vary in their speed, technical complexity, cost and ability to discriminate reliably between strains, with many of the recently developed methods targeting the genome to provide differentiation. No single typing method is individually superior, and ideally a combination of techniques should be employed depending on the level of discrimination required and time or resources available. The aim of this review is to consider the relative merits of the available typing methodologies with particular emphasis on those which may find application in a diagnostic laboratory.
AB - The epidemiological investigation of Escherichia coli O157 is complicated by the lack of heterogeneity between strains responsible for the majority of cases of infection. As a consequence it is difficult to reliably cluster together an outbreak strain and differentiate it from other sporadically occurring isolates. The methods available for the subtyping of E. coli O157 vary in their speed, technical complexity, cost and ability to discriminate reliably between strains, with many of the recently developed methods targeting the genome to provide differentiation. No single typing method is individually superior, and ideally a combination of techniques should be employed depending on the level of discrimination required and time or resources available. The aim of this review is to consider the relative merits of the available typing methodologies with particular emphasis on those which may find application in a diagnostic laboratory.
KW - AFLP
KW - epidemiological typing
KW - Escherichia coli O157
KW - molecular epidemiology
KW - PFGE
KW - RAPD
KW - sub-typing
KW - typing methods
KW - VTEC
UR - http://www.scopus.com/inward/record.url?scp=0034459857&partnerID=8YFLogxK
UR - https://link.springer.com/article/10.1023/A:1008941225987
U2 - 10.1023/A:1008941225987
DO - 10.1023/A:1008941225987
M3 - Article
SN - 1573-0972
VL - 16
SP - 741
EP - 748
JO - World Journal of Microbiology and Biotechnology
JF - World Journal of Microbiology and Biotechnology
IS - 8-9
ER -