Microarray analysis of expression of cell death-associated genes in spinal cord cells with cyclic tensile strain

Kenzo Uchida; Hideaki Nakajima; Takayuki Hirai; Sally Roberts; William E.B. Johnson; Hisatoshi Baba

doi:10.1007/978-4-431-54502-6_11

Microarray analysis of expression of cell death-associated genes in spinal cord cells with cyclic tensile strain

Kenzo Uchida^*, Hideaki Nakajima, Takayuki Hirai, Sally Roberts, William E.B. Johnson, Hisatoshi Baba

^*Corresponding author for this work

Research output: Chapter in Book/Published conference output › Chapter (peer-reviewed) › peer-review

Abstract

Previous studies have described alterations in gene expression following spinal cord injury, but this response to mechanical stimuli is difficult to investigate in vivo. Therefore, we have investigated the effect of cyclic tensile strain on cultured spinal cord cells from E15 Sprague-Dawley rats. Microarray analysis of gene expression and categorization of identified genes were performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) systems. The application of cyclic tensile strain reduced the viability of cultured spinal cord cells significantly in a dose- and time-dependent manner. GO analysis identified candidate genes related to apoptosis (44) and to response to stimulus (17). KEGG analysis identified changes in the expression levels of 12 genes of the mitogen-activated protein kinase (MAPK) signaling pathway, which were confirmed to be upregulated and validated by RT-PCR analysis. Spinal cord cells undergo cell death in response to cyclic tensile strain, which were dose- and time-dependent, with upregulation of various genes, in particular of the MAPK pathway.

Original language	English
Title of host publication	Neuroprotection and regeneration of the spinal cord
Editors	Kenzo Uchida, Masaya Nakamura, Hiroshi Ozawa, et al
Place of Publication	Tokyo (JP)
Publisher	Springer
Pages	119-127
Number of pages	9
ISBN (Electronic)	978-4-431-54502-6
ISBN (Print)	978-4-431-54501-9
DOIs	https://doi.org/10.1007/978-4-431-54502-6_11
Publication status	Published - 31 Jan 2014

Keywords

cultured cell
cyclic tensile strain
microarray
mitogen-activated protein kinase (MAPK) signaling pathway
spinal cord

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10.1007/978-4-431-54502-6_11

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Uchida, K., Nakajima, H., Hirai, T., Roberts, S., Johnson, W. E. B., & Baba, H. (2014). Microarray analysis of expression of cell death-associated genes in spinal cord cells with cyclic tensile strain. In K. Uchida, M. Nakamura, H. Ozawa, & et al (Eds.), Neuroprotection and regeneration of the spinal cord (pp. 119-127). Springer. https://doi.org/10.1007/978-4-431-54502-6_11

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abstract = "Previous studies have described alterations in gene expression following spinal cord injury, but this response to mechanical stimuli is difficult to investigate in vivo. Therefore, we have investigated the effect of cyclic tensile strain on cultured spinal cord cells from E15 Sprague-Dawley rats. Microarray analysis of gene expression and categorization of identified genes were performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) systems. The application of cyclic tensile strain reduced the viability of cultured spinal cord cells significantly in a dose- and time-dependent manner. GO analysis identified candidate genes related to apoptosis (44) and to response to stimulus (17). KEGG analysis identified changes in the expression levels of 12 genes of the mitogen-activated protein kinase (MAPK) signaling pathway, which were confirmed to be upregulated and validated by RT-PCR analysis. Spinal cord cells undergo cell death in response to cyclic tensile strain, which were dose- and time-dependent, with upregulation of various genes, in particular of the MAPK pathway.",

keywords = "cultured cell, cyclic tensile strain, microarray, mitogen-activated protein kinase (MAPK) signaling pathway, spinal cord",

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Microarray analysis of expression of cell death-associated genes in spinal cord cells with cyclic tensile strain. / Uchida, Kenzo; Nakajima, Hideaki; Hirai, Takayuki et al.
Neuroprotection and regeneration of the spinal cord. ed. / Kenzo Uchida; Masaya Nakamura; Hiroshi Ozawa; et al. Tokyo (JP): Springer, 2014. p. 119-127.

Research output: Chapter in Book/Published conference output › Chapter (peer-reviewed) › peer-review

TY - CHAP

T1 - Microarray analysis of expression of cell death-associated genes in spinal cord cells with cyclic tensile strain

AU - Uchida, Kenzo

AU - Nakajima, Hideaki

AU - Hirai, Takayuki

AU - Roberts, Sally

AU - Johnson, William E.B.

AU - Baba, Hisatoshi

PY - 2014/1/31

Y1 - 2014/1/31

N2 - Previous studies have described alterations in gene expression following spinal cord injury, but this response to mechanical stimuli is difficult to investigate in vivo. Therefore, we have investigated the effect of cyclic tensile strain on cultured spinal cord cells from E15 Sprague-Dawley rats. Microarray analysis of gene expression and categorization of identified genes were performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) systems. The application of cyclic tensile strain reduced the viability of cultured spinal cord cells significantly in a dose- and time-dependent manner. GO analysis identified candidate genes related to apoptosis (44) and to response to stimulus (17). KEGG analysis identified changes in the expression levels of 12 genes of the mitogen-activated protein kinase (MAPK) signaling pathway, which were confirmed to be upregulated and validated by RT-PCR analysis. Spinal cord cells undergo cell death in response to cyclic tensile strain, which were dose- and time-dependent, with upregulation of various genes, in particular of the MAPK pathway.

AB - Previous studies have described alterations in gene expression following spinal cord injury, but this response to mechanical stimuli is difficult to investigate in vivo. Therefore, we have investigated the effect of cyclic tensile strain on cultured spinal cord cells from E15 Sprague-Dawley rats. Microarray analysis of gene expression and categorization of identified genes were performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) systems. The application of cyclic tensile strain reduced the viability of cultured spinal cord cells significantly in a dose- and time-dependent manner. GO analysis identified candidate genes related to apoptosis (44) and to response to stimulus (17). KEGG analysis identified changes in the expression levels of 12 genes of the mitogen-activated protein kinase (MAPK) signaling pathway, which were confirmed to be upregulated and validated by RT-PCR analysis. Spinal cord cells undergo cell death in response to cyclic tensile strain, which were dose- and time-dependent, with upregulation of various genes, in particular of the MAPK pathway.

KW - cultured cell

KW - cyclic tensile strain

KW - microarray

KW - mitogen-activated protein kinase (MAPK) signaling pathway

KW - spinal cord

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BT - Neuroprotection and regeneration of the spinal cord

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ER -

Microarray analysis of expression of cell death-associated genes in spinal cord cells with cyclic tensile strain

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Keywords

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Blockade of interleukin-6 effects on cytokine profiles and macrophage activation after spinal cord injury in mice

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