Microarray analysis of expression of cell death-associated genes in spinal cord cells with cyclic tensile strain

Kenzo Uchida*, Hideaki Nakajima, Takayuki Hirai, Sally Roberts, William E.B. Johnson, Hisatoshi Baba

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Abstract

Previous studies have described alterations in gene expression following spinal cord injury, but this response to mechanical stimuli is difficult to investigate in vivo. Therefore, we have investigated the effect of cyclic tensile strain on cultured spinal cord cells from E15 Sprague-Dawley rats. Microarray analysis of gene expression and categorization of identified genes were performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) systems. The application of cyclic tensile strain reduced the viability of cultured spinal cord cells significantly in a dose- and time-dependent manner. GO analysis identified candidate genes related to apoptosis (44) and to response to stimulus (17). KEGG analysis identified changes in the expression levels of 12 genes of the mitogen-activated protein kinase (MAPK) signaling pathway, which were confirmed to be upregulated and validated by RT-PCR analysis. Spinal cord cells undergo cell death in response to cyclic tensile strain, which were dose- and time-dependent, with upregulation of various genes, in particular of the MAPK pathway.

Original languageEnglish
Title of host publicationNeuroprotection and regeneration of the spinal cord
EditorsKenzo Uchida, Masaya Nakamura, Hiroshi Ozawa, et al
Place of PublicationTokyo (JP)
PublisherSpringer
Pages119-127
Number of pages9
ISBN (Electronic)978-4-431-54502-6
ISBN (Print)978-4-431-54501-9
DOIs
Publication statusPublished - 31 Jan 2014

Keywords

  • cultured cell
  • cyclic tensile strain
  • microarray
  • mitogen-activated protein kinase (MAPK) signaling pathway
  • spinal cord

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  • Research Output

    • 1 Chapter (peer-reviewed)

    Blockade of interleukin-6 effects on cytokine profiles and macrophage activation after spinal cord injury in mice

    Rodriguez Guerrero, A., Uchida, K., Nakajima, H., Watanabe, S., Nakamura, M., Okada, S., Johnson, W. E. B. & Baba, H., 31 Jan 2014, Neuroprotection and regeneration of the spinal cord. Uchida, K., Nakamura, M., Ozawa, H. & et al (eds.). Tokyo (JP): Springer, p. 203-212 10 p.

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

  • Cite this

    Uchida, K., Nakajima, H., Hirai, T., Roberts, S., Johnson, W. E. B., & Baba, H. (2014). Microarray analysis of expression of cell death-associated genes in spinal cord cells with cyclic tensile strain. In K. Uchida, M. Nakamura, H. Ozawa, & et al (Eds.), Neuroprotection and regeneration of the spinal cord (pp. 119-127). Springer. https://doi.org/10.1007/978-4-431-54502-6_11