Microarray analysis of expression of cell death-associated genes in rat spinal cord cells exposed to cyclic tensile stresses in vitro

Kenzo Uchida, Hideaki Nakajima, Takayuki Hirai, Takafumi Yayama, Ke-Bing Chen, Shigeru Kobayashi, Sally Roberts, William E Johnson, Hisatoshi Baba

Research output: Contribution to journalArticle

Abstract

The application of mechanical insults to the spinal cord results in profound cellular and molecular changes, including the induction of neuronal cell death and altered gene expression profiles. Previous studies have described alterations in gene expression following spinal cord injury, but the specificity of this response to mechanical stimuli is difficult to investigate in vivo. Therefore, we have investigated the effect of cyclic tensile stresses on cultured spinal cord cells from E15 Sprague-Dawley rats, using the FX3000 Flexercell Strain Unit. We examined cell morphology and viability over a 72 hour time course. Microarray analysis of gene expression was performed using the Affymetrix GeneChip System, where categorization of identified genes was performed using the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) systems. Changes in expression of 12 genes were validated with quantitative real-time reverse transcription polymerase chain reaction (RT-PCR).
Original languageEnglish
Article number84
JournalBMC Nuroscience
Volume11
DOIs
Publication statusPublished - 22 Jul 2010

Bibliographical note

© 2010 Uchida et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords

  • animals
  • apoptosis
  • cultured cells
  • cluster analysis
  • gene expression profiling
  • MAP kinase signaling system
  • neurons
  • oligonucleotide array sequence analysis
  • rats
  • Sprague-Dawley rats
  • reverse transcriptase polymerase chain reaction
  • spinal cord
  • mechanical stress
  • physiological stress
  • time factors
  • up-regulation

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    Uchida, K., Nakajima, H., Hirai, T., Yayama, T., Chen, K-B., Kobayashi, S., Roberts, S., Johnson, W. E., & Baba, H. (2010). Microarray analysis of expression of cell death-associated genes in rat spinal cord cells exposed to cyclic tensile stresses in vitro. BMC Nuroscience, 11, [84]. https://doi.org/10.1186/1471-2202-11-84