Microfluidic-controlled manufacture of liposomes for the solubilisation of a poorly water soluble drug

Elisabeth Kastner, Varun Verma, Deborah Lowry, Yvonne Perrie

Research output: Contribution to journalArticle

Abstract

Besides their well-described use as delivery systems for water-soluble drugs, liposomes have the ability to act as a solubilizing agent for drugs with low aqueous solubility. However, a key limitation in exploiting liposome technology is the availability of scalable, low-cost production methods for the preparation of liposomes. Here we describe a new method, using microfluidics, to prepare liposomal solubilising systems which can incorporate low solubility drugs (in this case propofol). The setup, based on a chaotic advection micromixer, showed high drug loading (41 mol%) of propofol as well as the ability to manufacture vesicles with at prescribed sizes (between 50 and 450 nm) in a high-throughput setting. Our results demonstrate the ability of merging liposome manufacturing and drug encapsulation in a single process step, leading to an overall reduced process time. These studies emphasise the flexibility and ease of applying lab-on-a-chip microfluidics for the solubilisation of poorly water-soluble drugs.

LanguageEnglish
Pages122-130
Number of pages9
JournalInternational Journal of Pharmaceutics
Volume485
Issue number1-2
Early online date25 Feb 2015
DOIs
Publication statusPublished - 15 May 2015

Fingerprint

Microfluidics
Liposomes
Water
Pharmaceutical Preparations
Propofol
Solubility
Technology
Costs and Cost Analysis

Bibliographical note

NOTICE: this is the author’s version of a work that was accepted for publication in International journal of pharmaceutics. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Kastner, E, Verma, V, Lowry, D & Perrie, Y, 'Microfluidic-controlled manufacture of liposomes for the solubilisation of a poorly water soluble drug' International journal of pharmaceutics, vol 485, no. 1-2 (2015) DOI Kastner, E., Verma, V., Lowry, D., & Perrie, Y. (2015). Microfluidic-controlled manufacture of liposomes for the solubilisation of a poorly water soluble drug. International journal of pharmaceutics, 485(1-2), 122-130.

Partial funding: EPSRC

Keywords

  • bilayer loading
  • high throughput
  • liposomes
  • microfluidics
  • poorly soluble drugs

Cite this

Kastner, Elisabeth ; Verma, Varun ; Lowry, Deborah ; Perrie, Yvonne. / Microfluidic-controlled manufacture of liposomes for the solubilisation of a poorly water soluble drug. In: International Journal of Pharmaceutics. 2015 ; Vol. 485, No. 1-2. pp. 122-130.
@article{80462f4507f84088b78fbd456f986f54,
title = "Microfluidic-controlled manufacture of liposomes for the solubilisation of a poorly water soluble drug",
abstract = "Besides their well-described use as delivery systems for water-soluble drugs, liposomes have the ability to act as a solubilizing agent for drugs with low aqueous solubility. However, a key limitation in exploiting liposome technology is the availability of scalable, low-cost production methods for the preparation of liposomes. Here we describe a new method, using microfluidics, to prepare liposomal solubilising systems which can incorporate low solubility drugs (in this case propofol). The setup, based on a chaotic advection micromixer, showed high drug loading (41 mol{\%}) of propofol as well as the ability to manufacture vesicles with at prescribed sizes (between 50 and 450 nm) in a high-throughput setting. Our results demonstrate the ability of merging liposome manufacturing and drug encapsulation in a single process step, leading to an overall reduced process time. These studies emphasise the flexibility and ease of applying lab-on-a-chip microfluidics for the solubilisation of poorly water-soluble drugs.",
keywords = "bilayer loading, high throughput, liposomes, microfluidics, poorly soluble drugs",
author = "Elisabeth Kastner and Varun Verma and Deborah Lowry and Yvonne Perrie",
note = "NOTICE: this is the author’s version of a work that was accepted for publication in International journal of pharmaceutics. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Kastner, E, Verma, V, Lowry, D & Perrie, Y, 'Microfluidic-controlled manufacture of liposomes for the solubilisation of a poorly water soluble drug' International journal of pharmaceutics, vol 485, no. 1-2 (2015) DOI Kastner, E., Verma, V., Lowry, D., & Perrie, Y. (2015). Microfluidic-controlled manufacture of liposomes for the solubilisation of a poorly water soluble drug. International journal of pharmaceutics, 485(1-2), 122-130. Partial funding: EPSRC",
year = "2015",
month = "5",
day = "15",
doi = "10.1016/j.ijpharm.2015.02.063",
language = "English",
volume = "485",
pages = "122--130",
journal = "International Journal of Pharmaceutics",
issn = "0378-5173",
publisher = "Elsevier",
number = "1-2",

}

Microfluidic-controlled manufacture of liposomes for the solubilisation of a poorly water soluble drug. / Kastner, Elisabeth; Verma, Varun; Lowry, Deborah; Perrie, Yvonne.

In: International Journal of Pharmaceutics, Vol. 485, No. 1-2, 15.05.2015, p. 122-130.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Microfluidic-controlled manufacture of liposomes for the solubilisation of a poorly water soluble drug

AU - Kastner, Elisabeth

AU - Verma, Varun

AU - Lowry, Deborah

AU - Perrie, Yvonne

N1 - NOTICE: this is the author’s version of a work that was accepted for publication in International journal of pharmaceutics. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Kastner, E, Verma, V, Lowry, D & Perrie, Y, 'Microfluidic-controlled manufacture of liposomes for the solubilisation of a poorly water soluble drug' International journal of pharmaceutics, vol 485, no. 1-2 (2015) DOI Kastner, E., Verma, V., Lowry, D., & Perrie, Y. (2015). Microfluidic-controlled manufacture of liposomes for the solubilisation of a poorly water soluble drug. International journal of pharmaceutics, 485(1-2), 122-130. Partial funding: EPSRC

PY - 2015/5/15

Y1 - 2015/5/15

N2 - Besides their well-described use as delivery systems for water-soluble drugs, liposomes have the ability to act as a solubilizing agent for drugs with low aqueous solubility. However, a key limitation in exploiting liposome technology is the availability of scalable, low-cost production methods for the preparation of liposomes. Here we describe a new method, using microfluidics, to prepare liposomal solubilising systems which can incorporate low solubility drugs (in this case propofol). The setup, based on a chaotic advection micromixer, showed high drug loading (41 mol%) of propofol as well as the ability to manufacture vesicles with at prescribed sizes (between 50 and 450 nm) in a high-throughput setting. Our results demonstrate the ability of merging liposome manufacturing and drug encapsulation in a single process step, leading to an overall reduced process time. These studies emphasise the flexibility and ease of applying lab-on-a-chip microfluidics for the solubilisation of poorly water-soluble drugs.

AB - Besides their well-described use as delivery systems for water-soluble drugs, liposomes have the ability to act as a solubilizing agent for drugs with low aqueous solubility. However, a key limitation in exploiting liposome technology is the availability of scalable, low-cost production methods for the preparation of liposomes. Here we describe a new method, using microfluidics, to prepare liposomal solubilising systems which can incorporate low solubility drugs (in this case propofol). The setup, based on a chaotic advection micromixer, showed high drug loading (41 mol%) of propofol as well as the ability to manufacture vesicles with at prescribed sizes (between 50 and 450 nm) in a high-throughput setting. Our results demonstrate the ability of merging liposome manufacturing and drug encapsulation in a single process step, leading to an overall reduced process time. These studies emphasise the flexibility and ease of applying lab-on-a-chip microfluidics for the solubilisation of poorly water-soluble drugs.

KW - bilayer loading

KW - high throughput

KW - liposomes

KW - microfluidics

KW - poorly soluble drugs

UR - http://www.scopus.com/inward/record.url?scp=84924943296&partnerID=8YFLogxK

U2 - 10.1016/j.ijpharm.2015.02.063

DO - 10.1016/j.ijpharm.2015.02.063

M3 - Article

VL - 485

SP - 122

EP - 130

JO - International Journal of Pharmaceutics

T2 - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

SN - 0378-5173

IS - 1-2

ER -